The Effects of Platelet-Rich and Platelet-Poor Plasma on Biological Characteristics of BM-MSCs In Vitro
المؤلفون المشاركون
Zhang, Jiahui
Zhang, Jun
Zhang, Nannan
Li, Tao
Zhou, Xiaohe
Jia, Jue
Liang, Yingying
Sun, Xiaochun
Chen, Huabiao
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-26
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Platelet-rich plasma (PRP) and its byproduct platelet-poor plasma (PPP) are rich sources of cytokines in tissue damage repair.
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have received more and more attention for their ability to treat multiple diseases.
The purpose of our study was to investigate the biologic action of PPP and PRP on BM-MSCs.
The adipogenic potential of BM-MSCs revealed no obvious change, but the osteogenic ability of BM-MSCs was enhanced after treated with PRP.
CCK8 assays and cell colony formation assays showed that PRP promoted cell proliferation, while this effect of PPP was not obvious.
No obvious difference was found in cell cycle and apoptosis of BM-MSCs between PRP and PPP treatment.
Expression of β-galactosidase, a biological marker of senescence, was decreased upon PRP treatment which indicated that PRP provided significant protection against cellular senescence.
The migratory capacity of BM-MSCs was detected by scratch and transwell assays.
The results indicated that PRP could affect the migration ability of BM-MSCs.
From immunofluorescence detection and western blot, we demonstrated that the level of epithelial-mesenchymal transition-related proteins was changed and several pluripotency marker genes, including Sox2, Sall4, Oct4, and Nanog, were increased.
Finally, the expression of the key signal pathway such as PI3K/AKT was examined.
Our findings suggested that PRP promoted cell migration of BM-MSCs via stimulating the signaling pathway of PI3K/AKT.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Jiahui& Zhang, Jun& Zhang, Nannan& Li, Tao& Zhou, Xiaohe& Jia, Jue…[et al.]. 2020. The Effects of Platelet-Rich and Platelet-Poor Plasma on Biological Characteristics of BM-MSCs In Vitro. Analytical Cellular Pathology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1126205
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Jiahui…[et al.]. The Effects of Platelet-Rich and Platelet-Poor Plasma on Biological Characteristics of BM-MSCs In Vitro. Analytical Cellular Pathology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1126205
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Jiahui& Zhang, Jun& Zhang, Nannan& Li, Tao& Zhou, Xiaohe& Jia, Jue…[et al.]. The Effects of Platelet-Rich and Platelet-Poor Plasma on Biological Characteristics of BM-MSCs In Vitro. Analytical Cellular Pathology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1126205
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126205
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر