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Cell Fragmentation and Permeabilization by a 1 ns Pulse Driven Triple-Point Electrode
المؤلفون المشاركون
Cho, M.
Yang, Enbo
Li, Joy
Xiao, Shu
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Ultrashort electric pulses (ns-ps) are useful in gaining understanding as to how pulsed electric fields act upon biological cells, but the electric field intensity to induce biological responses is typically higher than longer pulses and therefore a high voltage ultrashort pulse generator is required.
To deliver 1 ns pulses with sufficient electric field but at a relatively low voltage, we used a glass-encapsulated tungsten wire triple-point electrode (TPE) at the interface among glass, tungsten wire, and water when it is immersed in water.
A high electric field (2 MV/cm) can be created when pulses are applied.
However, such a high electric field was found to cause bubble emission and temperature rise in the water near the electrode.
They can be attributed to Joule heating near the electrode.
Adherent cells on a cover slip treated by the combination of these stimuli showed two major effects: (1) cells in a crater (<100 μm from electrode) were fragmented and the debris was blown away.
The principal mechanism for the damage is presumed to be shear forces due to bubble collapse; and (2) cells in the periphery of the crater were permeabilized, which was due to the combination of bubble movement and microstreaming as well as pulsed electric fields.
These results show that ultrashort electric fields assisted by microbubbles can cause significant cell response and therefore a triple-point electrode is a useful ablation tool for applications that require submillimeter precision.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Enbo& Li, Joy& Cho, M.& Xiao, Shu. 2018. Cell Fragmentation and Permeabilization by a 1 ns Pulse Driven Triple-Point Electrode. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126336
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Enbo…[et al.]. Cell Fragmentation and Permeabilization by a 1 ns Pulse Driven Triple-Point Electrode. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126336
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Enbo& Li, Joy& Cho, M.& Xiao, Shu. Cell Fragmentation and Permeabilization by a 1 ns Pulse Driven Triple-Point Electrode. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126336
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126336
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