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Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-85-FU Cells via TOP2α and miR-494
المؤلفون المشاركون
Lin, Jiumao
Zhao, Jinyan
Peng, Jun
Fang, Yi
Yang, Chi
Zhang, Ling
Wei, Lihui
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The use of 5-fluorouracil (5-FU) has been proven benefits, but it also has adverse events in colorectal cancer (CRC) chemotherapy.
In this study, we explored the mechanism of 5-FU resistance by bioinformatics analysis of the NCBI public dataset series GSE81005.
Fifteen hub genes were screened out of 582 different expressed genes.
Modules of the hub genes in protein-protein interaction networks gathered to TOP2α showed a decrease in HCT-8 cells but an increase in 5-FU-resistant HCT-8/5-FU cells with 5-FU exposure.
Downregulation of TOP2α with siRNA or miR-494 transfection resulted in an increase of cytotoxicity and decrease of cell colonies to 5-FU for HCT-8/5-FU cells.
Moreover, we found that an ethanol extract of Spica Prunellae (EESP), which is a traditional Chinese medicine with clinically beneficial effects in various cancers, was able to enhance the sensitivity of 5-FU in HCT-8/5-FU cells and partly reverse the 5-FU resistance effect.
It significantly helped suppress cell growth and induced cell apoptosis in HCT-8/5-FU cells with the expression of TOP2α being significantly suppressed, which increased by 5-FU.
Consistently, miR-494, which reportedly regulates TOP2α, exhibited reverse trends in EESP/5-FU combination treatment.
These results suggested that Spica Prunellae may be beneficial in the treatment of 5-FU-resistant CRC patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fang, Yi& Yang, Chi& Zhang, Ling& Wei, Lihui& Lin, Jiumao& Zhao, Jinyan…[et al.]. 2019. Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-85-FU Cells via TOP2α and miR-494. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126349
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fang, Yi…[et al.]. Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-85-FU Cells via TOP2α and miR-494. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1126349
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fang, Yi& Yang, Chi& Zhang, Ling& Wei, Lihui& Lin, Jiumao& Zhao, Jinyan…[et al.]. Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-85-FU Cells via TOP2α and miR-494. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126349
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126349
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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