Local Tensile Stress in the Development of Posttraumatic Osteoarthritis

الملخص EN

The pathogenesis of posttraumatic osteoarthritis (PTOA) remains unrevealed.

We speculate that cartilage crack caused by joint trauma will induce local abnormal tensile stress, leading to change in extracellular matrix (ECM) expression of chondrocytes, cartilage degeneration, and initiation of osteoarthritis.

Finite element model was used to examine whether the local tensile stress could be produced around the crack.

Cell experiments were conducted to test the effect of tensile strain on chondrocyte ECM expression.

Animal tests in rabbits were carried out to examine the change around the cartilage crack.

The results indicated that the local tensile stress was generated around the crack and varied with the crack angles.

The maximum principal tensile stress was 0.59 MPa around the 45° crack, and no tensile stress was found at 90°.

10% tensile strain could significantly promote type I collagen mRNA expression and inhibit type II collagen and aggrecan (the proteoglycan core protein) mRNA expression.

Type I collagen was detected around the 45° crack region in the cartilage with no change in type II collagen and proteoglycan.

We conclude that the local tensile stress produced around the cartilage crack can cause the change in cartilage matrix expression which might lead to cartilage degeneration and initiation of osteoarthritis.

This study provides biomechanical-based insight into the pathogenesis of PTOA and potentially new intervention in prevention and treatment of PTOA.