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Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma
المؤلفون المشاركون
Li, Yi
Lu, Jinying
Bai, Furong
Xiao, Yanan
Guo, Yiran
Dong, Ziming
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-19
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Osteosarcoma is the most common primary malignancy of bone in children and the elderly.
Recently, more and more researches have demonstrated that Ginsenoside Rg3 (Rg3) is involved in chemotherapy resistance in many cancer, making it a promising Chinese herbal monomer for oncotherapy.
In this study, we investigated the efficacy of Rg3 in human osteosarcoma cell lines (MG-63, U-2OS, and SaOS-2).
Cell proliferation was measured by CCK8 assay.
The migration of cells was examined using the scratch assay method.
Quantification of apoptosis was assessed further by flow cytometry.
In addition, the expression of apoptosis-related genes (caspase9, caspase3, Bcl2, and Bax) were investigated using RT-PCR.
We further investigated the protein level expression of Bcl 2, cleaved-caspase3, and PI3K/AKT/mTOR signaling pathway factors by Western blot assay.
Our results revealed that Rg3 inhibited the proliferation and migration of human osteosarcoma cells and induced apoptosis in a concentration- and time-dependent manner.
Western blot results showed that Rg3 reduced the protein expression of Bcl2 and PI3K/AKT/mTORbut increased the levels of cleaved-caspase3.
Therefore, we hypothesized Rg3 inhibits the proliferation of osteosarcoma cell line and induces their apoptosis by affecting apoptosis-related genes (Bcl2, caspase3) as well as the PI3K/AKT/mTOR signaling pathway.
To conclude, Rg3 is a new therapeutic agent against osteosarcoma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Yi& Lu, Jinying& Bai, Furong& Xiao, Yanan& Guo, Yiran& Dong, Ziming. 2018. Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126526
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Yi…[et al.]. Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1126526
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Yi& Lu, Jinying& Bai, Furong& Xiao, Yanan& Guo, Yiran& Dong, Ziming. Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126526
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126526
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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