Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans
المؤلفون المشاركون
Li, Bingchun
Li, Xinlong
Lin, Huancai
Zhou, Yan
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-28
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Streptococcus mutans (S.
mutans) has been proved to be the main aetiological factor in dental caries.
Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest.
The objective of this study is to evaluate the inhibitory effects of curcumin on metabolism and biofilm formation in S.
mutans using a vitro biofilm model in an artificial oral environment.
S.
mutans biofilms were treated with varying concentrations of curcumin.
The biofilm metabolism and biofilm biomass were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the crystal violet assay.
Confocal laser scanning microscopy was used to analyse the composition and extracellular polysaccharide content of S.
mutans biofilm after curcumin treatment.
The biofilm structure was evaluated using a scanning electron microscope.
The gene expression of virulence-related factors was assessed by real-time PCR.
The antibiofilm effect of curcumin was compared with that of chlorhexidine.
The sessile minimum inhibitory concentration (SMIC50%) of curcumin against S.
mutans biofilm was 500 μM.
Curcumin reduced the biofilm metabolism from 5 min to 24 h.
Curcumin inhibited the quantity of live bacteria and total bacteria in both the short term (5 min) and the long term.
Moreover, curcumin decreased the production of extracellular polysaccharide in the short term.
The expression of genes related to extracellular polysaccharide synthesis, carbohydrate metabolism, adherence, and the two-component transduction system decreased after curcumin treatment.
The chlorhexidine-treated group showed similar results.
We speculate that curcumin has the capacity to be developed as an alternative agent with the potential to reduce the pathogenic traits of S.
mutans biofilm.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Bingchun& Li, Xinlong& Lin, Huancai& Zhou, Yan. 2018. Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126662
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Bingchun…[et al.]. Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1126662
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Bingchun& Li, Xinlong& Lin, Huancai& Zhou, Yan. Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126662
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126662
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر