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miR-221 Augments TRAIL-Mediated Apoptosis in Prostate Cancer Cells by Inducing Endogenous TRAIL Expression and Targeting the Functional Repressors SOCS3 and PIK3R1
المؤلفون المشاركون
Zoni, Eugenio
Kruithof-de Julio, Marianna
Krebs, Markus
Behrmann, Christoph
Kalogirou, Charis
Sokolakis, Ioannis
Kneitz, Susanne
Rech, Anne
Schilling, Bastian
Kübler, Hubert
Spahn, Martin
Kneitz, Burkhard
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-14
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
miR-221 is regarded as an oncogene in many malignancies, and miR-221-mediated resistance towards TRAIL was one of the first oncogenic roles shown for this small noncoding RNA.
In contrast, miR-221 is downregulated in prostate cancer (PCa), thereby implying a tumour suppressive function.
By using proliferation and apoptosis assays, we show a novel feature of miR-221 in PCa cells: instead of inducing TRAIL resistance, miR-221 sensitized cells towards TRAIL-induced proliferation inhibition and apoptosis induction.
Partially responsible for this effect was the interferon-mediated gene signature, which among other things contained an endogenous overexpression of the TRAIL encoding gene TNFSF10.
This TRAIL-friendly environment was provoked by downregulation of the established miR-221 target gene SOCS3.
Moreover, we introduced PIK3R1 as a target gene of miR-221 in PCa cells.
Proliferation assays showed that siRNA-mediated downregulation of SOCS3 and PIK3R1 mimicked the effect of miR-221 on TRAIL sensitivity.
Finally, Western blotting experiments confirmed lower amounts of phospho-Akt after siRNA-mediated downregulation of PIK3R1 in PC3 cells.
Our results further support the tumour suppressing role of miR-221 in PCa, since it sensitises PCa cells towards TRAIL by regulating the expression of the oncogenes SOCS3 and PIK3R1.
Given the TRAIL-inhibiting effect of miR-221 in various cancer entities, our results suggest that the influence of miR-221 on TRAIL-mediated apoptosis is highly context- and entity-dependent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Krebs, Markus& Behrmann, Christoph& Kalogirou, Charis& Sokolakis, Ioannis& Kneitz, Susanne& Kruithof-de Julio, Marianna…[et al.]. 2019. miR-221 Augments TRAIL-Mediated Apoptosis in Prostate Cancer Cells by Inducing Endogenous TRAIL Expression and Targeting the Functional Repressors SOCS3 and PIK3R1. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1126723
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Krebs, Markus…[et al.]. miR-221 Augments TRAIL-Mediated Apoptosis in Prostate Cancer Cells by Inducing Endogenous TRAIL Expression and Targeting the Functional Repressors SOCS3 and PIK3R1. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1126723
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Krebs, Markus& Behrmann, Christoph& Kalogirou, Charis& Sokolakis, Ioannis& Kneitz, Susanne& Kruithof-de Julio, Marianna…[et al.]. miR-221 Augments TRAIL-Mediated Apoptosis in Prostate Cancer Cells by Inducing Endogenous TRAIL Expression and Targeting the Functional Repressors SOCS3 and PIK3R1. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1126723
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126723
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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