Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model

المؤلفون المشاركون

Liu, Yang
Wang, Dong
Wu, Xueqiang
Zhou, Junlin

المصدر

BioMed Research International

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-04-16

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

To find a simple and noninvasive method to promote fracture healing, we are trying to explore whether repetitive brief ischemia would promote bone healing.

88 rats divided into 6 groups were used to make right tibia closed fracture caused by the heavy weight collision method.

Healthy side groups received homemade tourniquet placed on left and affected side group placed on right thigh 10 min inflated/10 min deflated 3 times every 24 hours or 48 hours after tibia fractured.

Rats in control groups received homemade tourniquet uninflated placed on right thigh 1 hour every 24 hours or 48 hours.

X-rays were checked at 1, 2, and 4 weeks.

Micro-CT inspected the bone healing at 2 and 4 weeks.

Serum cytokines, such as bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), diethanolamine enzyme activity unit of alkaline phosphatase (ALP) and transforming growth factor-β1 (TGF-β1), interleukin 10 (IL-10) and interleukin 6 (IL-6), were checked at 1, 2, and 4 weeks.

Local histology was evaluated at 2 weeks.

HE dye and BMP-2, VEGF, TGF-β, and ALP immunohistochemical stains were made.

Callus areas of posterior-anterior and lateral views were calculated and repetitive brief ischemia increased the callus areas ratio at 1 and 2 weeks.

Besides, from micro-CT results, repetitive brief ischemia increased the bone volume (BV) at 2 and 4 weeks and also increased the total bone tissue volume (TV) at 2 weeks and BV/TV at 4 weeks.

The serum cytokines, such as BMP-2, VEGF, diethanolamine enzyme activity unit of ALP and TGF-β1, have increased by repetitive brief ischemia at 1, 2 weeks.

It is opposite of affected side group that the level of serum IL-10 increased and IL-6 decreased in healthy side group at 1, 2 weeks.

Repetitive brief ischemia increased the callus area at 2 weeks and boosted the synthesis of BMP-2, VEGF, TGF-β, and ALP in the fracture region at 2 weeks from tissue stains.

Repetitive brief ischemia promotes bone healing no matter on the affected side or the healthy side limb.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Liu, Yang& Wang, Dong& Wu, Xueqiang& Zhou, Junlin. 2019. Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126854

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Liu, Yang…[et al.]. Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1126854

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Liu, Yang& Wang, Dong& Wu, Xueqiang& Zhou, Junlin. Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126854

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1126854