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Vascular Endothelial Growth Factor Is Regulated by the Canonical and Noncanonical Transforming Growth Factor-β Pathway in Synovial Fibroblasts Derived from Osteoarthritis Patients
المؤلفون المشاركون
Uchida, Kentaro
Aikawa, Jun
Takano, Shotaro
Sekiguchi, Hiroyuki
Shoji, Shintaro
Itakura, Makoto
Iwase, Dai
Mukai, Manabu
Takaso, Masashi
Inoue, Gen
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-29
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
Previous studies suggest the presence of an association of vascular endothelial growth factor (VEGF) with osteoarthritis (OA) severity and pain in patients with knee OA.
VEGF expression in human synovial fibroblasts (SFs) is induced by transforming growth factor-beta (TGFβ).
However, the signaling pathway governing TGFβ-mediated regulation of VEGF in SFs has not been identified.
Methods.
OA patients who underwent total knee arthroplasty had their synovial tissue (SYT) extracted and the constituent SFs cultured.
The cells were stimulated with culture medium (control), human recombinant TGFβ (hrTGFβ), hrTGFβ + ALK5 inhibitor SB505124, hrTGFβ + transforming growth factor activating kinase 1 (TAK1) inhibitor (5Z)-7-oxozeaenol, or hrTGFβ + p38 inhibitor SB203580 for 6 h.
VEGF mRNA expression in SFs was examined using real-time polymerase chain reaction and VEGF protein production in the cell supernatant was examined using enzyme-linked immunosorbent assay.
Additionally, phosphorylated levels of SMAD2 and p38 were examined using western blotting.
Results.
ALK5 (SB505124) and TAK1 (5Z-oxozeaenol) inhibitors completely suppressed TGFβ-induced VEGF mRNA expression and VEGF protein production.
Both SB505124 and 5Z-oxozeaenol also suppressed SMAD2 and p38 phosphorylation.
The p38 inhibitor (SB203580) partially inhibited TGFβ-mediated VEGF mRNA and VEGF protein production.
Conclusion.
TGFβ-mediated regulation of VEGF expression and VEGF protein production in the SYT of OA patients occurs through both the canonical and noncanonical pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Takano, Shotaro& Uchida, Kentaro& Shoji, Shintaro& Itakura, Makoto& Iwase, Dai& Aikawa, Jun…[et al.]. 2019. Vascular Endothelial Growth Factor Is Regulated by the Canonical and Noncanonical Transforming Growth Factor-β Pathway in Synovial Fibroblasts Derived from Osteoarthritis Patients. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1127035
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Takano, Shotaro…[et al.]. Vascular Endothelial Growth Factor Is Regulated by the Canonical and Noncanonical Transforming Growth Factor-β Pathway in Synovial Fibroblasts Derived from Osteoarthritis Patients. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1127035
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Takano, Shotaro& Uchida, Kentaro& Shoji, Shintaro& Itakura, Makoto& Iwase, Dai& Aikawa, Jun…[et al.]. Vascular Endothelial Growth Factor Is Regulated by the Canonical and Noncanonical Transforming Growth Factor-β Pathway in Synovial Fibroblasts Derived from Osteoarthritis Patients. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1127035
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1127035
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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