![](/images/graphics-bg.png)
Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
المؤلفون المشاركون
Cai, Yonglin
Liu, Fei
Wei, Jiazhang
Hao, Yanrong
Tang, Fengzhu
Jiao, Wei
Qu, Shenhong
He, Ning
Lan, Jiao
Yang, Yong
Wang, Yongli
Li, Min
Weng, Jingjin
Li, Bing
Lu, Jinlong
Han, Xing
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-11
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression.
However, molecular mechanisms of different levels of ZBTB7A are still unclear.
It is necessary to search molecular markers which are closely connected with ZBTB7A.
We selected NPC sublines CNE2 with stably transfecting empty plasmid (negative control, NC) and short hair RNA (shRNA) plasmid targeting ZBTB7A as research objectives.
Microarray was used to screen differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) via shRNA-CNE2 versus NC-CNE2.
Quantitative PCR (qPCR) was used to validate lncRNAs and mRNAs from the sublines, chronic rhinitis, and NPC tissues.
Bioinformatics was used to analyze regulatory pathways which were connected with ZBTB7A.
The 1501 lncRNAs (long noncoding RNAs) and 1275 differentially expressed mRNAs were upregulated or downregulated over 2-fold.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the upregulated or downregulated carbohydrate and lipid metabolisms probably involved in carcinogenicity of shRNA-CNE2 (P-value cut-off was 0.05).
In order to find the molecular mechanisms of ZBTB7A, we validated 12 differentially expressed lncRNAs and their nearby mRNAs by qPCR.
Most of the differentially expressed mRNAs are closely connected with carbohydrate and lipid metabolisms in multiply cancers.
Furthermore, part of them were validated in NPC and rhinitis tissues by qPCR.
As a result, NR_047538, ENST00000442852, and fatty acid synthase (FASN) were closely associated with NPC.
ZBTB7A had a positive association with NR_047538 and negative associations with ENST00000442852 and FASN.
The results probably provide novel candidate biomarkers for NPC progression with different levels of ZBTB7A.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Fei& Wei, Jiazhang& Hao, Yanrong& Tang, Fengzhu& Jiao, Wei& Qu, Shenhong…[et al.]. 2019. Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma. BioMed Research International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1127179
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Fei…[et al.]. Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma. BioMed Research International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1127179
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Fei& Wei, Jiazhang& Hao, Yanrong& Tang, Fengzhu& Jiao, Wei& Qu, Shenhong…[et al.]. Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1127179
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1127179
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)