γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway
المؤلفون المشاركون
Chen, Wanbing
Wu, You
Zhang, Gaofu
Wang, Mo
Yang, Haiping
Li, Qiu
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-15
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Primary nephrotic syndrome (PNS) is a devastating pediatric disorder.
However, its mechanism remains unclear.
Previous studies detected B7-1 in podocytes; meanwhile, γδT cells play pivotal roles in immune diseases.
Therefore, this study aimed to assess whether and how γδT cells impact podocytes via the CD28/B7-1 pathway.
WT and TCRδ−/− mice were assessed.
LPS was used to induce nephropathy.
Total γδT and CD28+γδT cells were quantitated in mouse spleen and kidney samples.
B7-1 and phosphor-SRC levels in the kidney were detected as well.
In vitro, γδT cells from the mouse spleen were cocultured with mouse podocytes, and apoptosis rate and phosphor-SRC expression in podocytes were assessed.
Compared with control mice, WT mice with LPS nephropathy showed increased amounts of γδT cells in the kidney.
Kidney injury was alleviated in TCRδ−/− mice.
Meanwhile, B7-1 and phosphor-SRC levels were increased in the kidney from WT mice with LPS nephropathy.
CD28+γδT cells were decreased, indicating CD28 may play a role in LPS nephropathy.
Immunofluorescence colocalization analysis revealed a tight association of γδT cells with B7-1 in the kidney.
High B7-1 expression was detected in podocytes treated with LPS.
Podocytes cocultured with γδT cells showed higher phosphor-SRC and apoptosis rate than other cell groups.
Furthermore, CD28/B7-1 blockage with CTLA4-Ig in vitro relieved podocyte injury.
γδT cells exacerbate podocyte injury via CD28/B7-1 signaling, with downstream involvement of phosphor-SRC.
The CD28/B7-1 blocker CTLA4-Ig prevented progressive podocyte injury, providing a potential therapeutic tool for PNS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Wanbing& Wu, You& Zhang, Gaofu& Wang, Mo& Yang, Haiping& Li, Qiu. 2018. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Wanbing…[et al.]. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Wanbing& Wu, You& Zhang, Gaofu& Wang, Mo& Yang, Haiping& Li, Qiu. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1127389
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر