γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway
Joint Authors
Chen, Wanbing
Wu, You
Zhang, Gaofu
Wang, Mo
Yang, Haiping
Li, Qiu
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Primary nephrotic syndrome (PNS) is a devastating pediatric disorder.
However, its mechanism remains unclear.
Previous studies detected B7-1 in podocytes; meanwhile, γδT cells play pivotal roles in immune diseases.
Therefore, this study aimed to assess whether and how γδT cells impact podocytes via the CD28/B7-1 pathway.
WT and TCRδ−/− mice were assessed.
LPS was used to induce nephropathy.
Total γδT and CD28+γδT cells were quantitated in mouse spleen and kidney samples.
B7-1 and phosphor-SRC levels in the kidney were detected as well.
In vitro, γδT cells from the mouse spleen were cocultured with mouse podocytes, and apoptosis rate and phosphor-SRC expression in podocytes were assessed.
Compared with control mice, WT mice with LPS nephropathy showed increased amounts of γδT cells in the kidney.
Kidney injury was alleviated in TCRδ−/− mice.
Meanwhile, B7-1 and phosphor-SRC levels were increased in the kidney from WT mice with LPS nephropathy.
CD28+γδT cells were decreased, indicating CD28 may play a role in LPS nephropathy.
Immunofluorescence colocalization analysis revealed a tight association of γδT cells with B7-1 in the kidney.
High B7-1 expression was detected in podocytes treated with LPS.
Podocytes cocultured with γδT cells showed higher phosphor-SRC and apoptosis rate than other cell groups.
Furthermore, CD28/B7-1 blockage with CTLA4-Ig in vitro relieved podocyte injury.
γδT cells exacerbate podocyte injury via CD28/B7-1 signaling, with downstream involvement of phosphor-SRC.
The CD28/B7-1 blocker CTLA4-Ig prevented progressive podocyte injury, providing a potential therapeutic tool for PNS.
American Psychological Association (APA)
Chen, Wanbing& Wu, You& Zhang, Gaofu& Wang, Mo& Yang, Haiping& Li, Qiu. 2018. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
Modern Language Association (MLA)
Chen, Wanbing…[et al.]. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
American Medical Association (AMA)
Chen, Wanbing& Wu, You& Zhang, Gaofu& Wang, Mo& Yang, Haiping& Li, Qiu. γδT Cells Exacerbate Podocyte Injury via the CD28B7-1-Phosphor-SRC Kinase Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1127389
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127389