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Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway
المؤلفون المشاركون
Zhou, Xiao-Long
Chen, Gang
Li, Meng-Xue
Wang, Heng-Xue
Hong, Jia-Wei
Shen, Jun-Yu
Wang, Qi
Ge, Xing
Ding, Zhen
Xu, Li-Chun
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
We have identified a gene YOD1 encoding deubiquitinating enzyme (DUB) responsible for nonsyndromic cleft lip with or without cleft palate (NSCL/P).
We aimed to determine the effects of YOD1 RNA interference (RNAi) on cell proliferation and migration, playing an important role in lip and palate formation, and to clarify whether the mechanisms involved TGF-β3 signaling associated with NSCL/P.
Methods.
RNAi was applied to construct vectors expressing YOD1 small interference RNAs (siRNAs).
The vectors were transfected into the human oral keratinocytes (HOK) cells.
The cell proliferation and migration were evaluated by the cell counting kit-8 (CCK-8) assay and wound healing assay, respectively.
The mRNA levels were detected by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR).
The protein levels were investigated by western blotting.
Results.
The proliferation of YOD1 siRNA-transfected HOK cells was remarkably inhibited.
The migration rate was significantly decreased in the YOD1 siRNA-transfected HOK cells.
The TGF-β3 mRNA and protein levels were decreased significantly by siRNA-mediated knockdown of YOD1.
YOD1 RNAi reduced the phosphor-Smad2/3 levels significantly.
Conclusions.
YOD1 RNAi may inhibit cell proliferation and migration associated with the pathogenesis of NSCL/P through TGF-β3 signaling.
The study indicates a novel role of YOD1 in regulating TGF-β3 signaling to affect cell proliferation and migration resulting in NSCL/P.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Xiao-Long& Chen, Gang& Li, Meng-Xue& Wang, Heng-Xue& Hong, Jia-Wei& Shen, Jun-Yu…[et al.]. 2018. Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127802
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Xiao-Long…[et al.]. Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1127802
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Xiao-Long& Chen, Gang& Li, Meng-Xue& Wang, Heng-Xue& Hong, Jia-Wei& Shen, Jun-Yu…[et al.]. Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127802
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1127802
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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