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Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids
المؤلفون المشاركون
Michal, Mikula
Jerzy, Ostrowski
Dabrowska, Michalina
Karczmarski, Jakub
Paziewska, Agnieszka
Polkowski, Marcin
Rubel, Tymon
Dadlez, Michal
Wiechowska-Kozłowska, Anna
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-29
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Pancreatic cyst fluids (PCFs) enriched in tumour-derived proteins are considered a potential source of new biomarkers.
This study aimed to determine compositional and quantitative differences between the degradome and proteome of PCFs aspirated from different types of pancreatic cyst lesions (PCLs).
91 patients who underwent endoscopic ultrasound-fine needle aspiration under routine clinical diagnosis of PCLs were enrolled.
Four cysts were malignant (CAs), and 87 were nonmalignant and consisted of 18 intraductal papillary mucinous neoplasms (IPMNs), 14 mucinous cystic neoplasms (MCNs), nine serous cystic neoplasms (SCNs), 29 pseudocysts (PCs), and 17 unclassified.
Profiles of the <5 kDa fraction, the degradome, and the trypsin-digested proteome were analysed using an LTQ-Orbitrap Elite mass spectrometer coupled with a nanoACQUITY LC system.
Qualitative analyses identified 796 and 366 proteins in degradome and proteome, respectively, and 689 (77%) and 285 (78%) of them were present in the Plasma Proteome Database.
Gene Ontology analysis showed a significant overrepresentation of peptidases and peptidases inhibitors in both datasets.
In the degradome fraction, quantitative values were obtained for 6996 peptides originating from 657 proteins.
Of these, 2287 peptides were unique to a single type, and 515 peptides, derived from 126 proteins, were shared across cyst types.
32 peptides originating from 12 proteins had differential (adjusted p-value ≤0.05, FC ≥1.5) abundance in at least one of the five cysts types.
In proteome, relative expression was measured for 330 proteins.
Of them, 33 proteins had significantly (adjusted p-value ≤0.05, FC ≥1.5) altered abundance in at least one of the studied groups and 19 proteins appeared to be unique to a given cyst type.
PCFs are dominated by blood proteins and proteolytic enzymes.
Although differences in PCF peptide composition and abundance could aid classification of PCLs, the unpredictable inherent PCF proteolytic activity may limit the practical applications of PCF protein profiling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Paziewska, Agnieszka& Polkowski, Marcin& Rubel, Tymon& Karczmarski, Jakub& Wiechowska-Kozłowska, Anna& Dabrowska, Michalina…[et al.]. 2018. Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128303
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Paziewska, Agnieszka…[et al.]. Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1128303
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Paziewska, Agnieszka& Polkowski, Marcin& Rubel, Tymon& Karczmarski, Jakub& Wiechowska-Kozłowska, Anna& Dabrowska, Michalina…[et al.]. Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128303
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128303
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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