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Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes
المؤلفون المشاركون
Jing, Kaipeng
Quan, Juanhua
Zhang, Man-Feng
Cai, Xiao-Lin
Pi, Xiao-Xue
Liao, Pei-Yu
Li, Shi-Jie
Wen-Li, Shi-Jie
Cai, Chuan-Chuan
Fan, Yi-Ming
Ge-Shi, Yi-Ming
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear.
We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins.
Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days.
Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation.
Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation.
Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture.
iTRAQ analysis identified 3582 expressed proteins in this differentiation model.
Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively.
Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Man-Feng& Cai, Xiao-Lin& Jing, Kaipeng& Pi, Xiao-Xue& Liao, Pei-Yu& Li, Shi-Jie…[et al.]. 2018. Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128305
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Man-Feng…[et al.]. Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1128305
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Man-Feng& Cai, Xiao-Lin& Jing, Kaipeng& Pi, Xiao-Xue& Liao, Pei-Yu& Li, Shi-Jie…[et al.]. Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128305
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128305
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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