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Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture
المؤلفون المشاركون
Michel, Olga
Kulbacka, Julita
Saczko, Jolanta
Mączyńska, Justyna
Błasiak, Piotr
Rossowska, Joanna
Rzechonek, Adam
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Despite the rapid progression of cancer pharmacotherapy, the high drug resistance of pancreatic ductal adenocarcinoma (PDA) makes it one of the most lethal malignancies.
Therefore, there are high expectations associated with experimental therapies, such as electrochemotherapy (ECT).
This technique involves the application of short electric pulses to induce transitional permeabilization of the cellular membrane, thus enhancing drug molecules influx.
The aim of the study was to investigate the influence of electroporation with cisplatin (CisEP) on the primary culture of human PDA cells from lung metastases—their survival and stress response.
Considering the growing importance of various research models, two established human PDA cell lines, EPP85-181P (sensitive to daunorubicin) and EPP85-181RDB (resistant to daunorubicin), were utilized as a reference control.
Cisplatin revealed higher cytotoxicity towards established cell lines.
Following CisEP application, we observed a significant decrease of cells viability in the primary culture model.
After CisEP therapy, an increased immunoreactivity with SOD-2 and Casp-3 antibodies was noticed.
In conclusion, we discovered that electroporation can enhance the cytotoxic effect of cisplatin in pancreatic cancer cells in vitro.
This effect was evident for cells from the primary culture.
The obtained results confirm the importance of primary cells models in studies on the efficacy of experimental cancer therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Michel, Olga& Kulbacka, Julita& Saczko, Jolanta& Mączyńska, Justyna& Błasiak, Piotr& Rossowska, Joanna…[et al.]. 2018. Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128431
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Michel, Olga…[et al.]. Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1128431
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Michel, Olga& Kulbacka, Julita& Saczko, Jolanta& Mączyńska, Justyna& Błasiak, Piotr& Rossowska, Joanna…[et al.]. Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128431
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128431
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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