![](/images/graphics-bg.png)
Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli
المؤلفون المشاركون
Ahmed, Syed Shoaib
Akhter, Mohsina
Sajjad, Muhammad
Gul, Roquyya
Khurshid, Sana
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-07
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
This study aims to achieve high-level soluble expression and characterization of a thermostable industrially important enzyme, i.e., beta-glucosidase (BglA; EC: 3.2.1.21), from Clostridium thermocellum (C.
thermocellum) by cloning in an Escherichia coli (E.
coli) expression system.
BglA was expressed as a partially soluble component of total cellular protein (TCP) having a molecular weight of ∼53 kDa with 50% of it as soluble fraction.
Purification in two steps, namely, heat inactivation and Ni-chromatography, yielded approximately 30% and 15% of BglA, respectively.
The purified (∼98%) BglA enzyme showed promising activity against the salicin substrate having a Km of 19.83 mM and a Vmax of 0.12 μmol/min.
The enzyme had an optimal temperature and pH of 50°C and 7.0, respectively, while retaining its catalytic activity up till 60°C and at pH 7.
The optimized maximum expression level was attained in M9NG medium with lactose as an inducer.
Circular dichroism revealed presence of alpha helix (43.50%) and small percentage of beta sheets (10.60%).
Factors like high-end cellulolytic activity, fair thermal stability, stability against low pH, and ease of purification make BglA from C.
thermocellum a potential candidate in industrial applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ahmed, Syed Shoaib& Akhter, Mohsina& Sajjad, Muhammad& Gul, Roquyya& Khurshid, Sana. 2019. Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128460
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ahmed, Syed Shoaib…[et al.]. Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128460
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ahmed, Syed Shoaib& Akhter, Mohsina& Sajjad, Muhammad& Gul, Roquyya& Khurshid, Sana. Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128460
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128460
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)