Identification and Characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile Isolates from Reptiles in Brazil
المؤلفون المشاركون
Heinemann, Marcos Bryan
Morcatti Coura, Fernanda
Silva, Rodrigo Otávio Silveira
Ramos, Carolina Pantuzza
Santana, Jordana Almeida
Xavier, Rafael Gariglio Clark
Leal, Carlos Augusto Gomes
Oliveira Junior, Carlos Augusto
Lage, Andrey Pereira
Lobato, Francisco Carlos Faria
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-27
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Considering the increasing popularity of reptiles as pets and their possible role as reservoirs of pathogenic microorganisms, the aim of this study was to isolate Escherichia coli, Salmonella spp., Clostridium perfringens, and C.
difficile strains from reptiles in Brazil and to characterize the isolated strains.
The characterization was based on phylogenetic typing of E.
coli, identification of virulence genes of E.
coli, C.
perfringens, and C.
difficile, serotyping of Salmonella spp., ribotyping and MLST of C.
difficile and antimicrobial susceptibility test of pathogenic strains.
Cloacal swabs were collected from 76 reptiles, of which 15 were lizards, 16 chelonians, and 45 snakes, either living in captivity, in the wild, or as companion animals.
E.
coli was isolated from 52 (68.4%) reptiles, of which 46 (88.4%) were characterized as phylogroup B1.
The virulence factor CNF1 of E.
coli was found in seven (9.2%) sampled animals, whereas the gene of EAST1 was found in isolates from two (2.6%) reptiles.
Three isolates positive for CNF1 were resistant to cephalothin, one of which was also resistant to ciprofloxacin, trimethoprim/sulfamethoxazole, and chloramphenicol, being then classified as multidrug resistant strain (MDR).
Salmonella enterica was identified in 26 (34.2%) reptiles, of which 13 belonged to the subspecies enterica.
Serotypes such as S.
Mbandaka, S.
Panama, S.
Infantis, S.
Heidelberg, and S.
Anatum were identified.
One isolate of S.
enterica subsp.
houtenae was resistant to cephalothin and ciprofloxacin.
C.
perfringens type A was isolated from six (7.8%) animals.
C.
difficile was isolated from three (3.9%) reptiles.
Two of these isolates were toxigenic and classified into ribotypes/MLST 081/ST9 and 106/ST42, which have been previously reported to infect humans.
In conclusion, reptiles in Brazil can harbor toxigenic C.
difficile and potentially pathogenic E.
coli and Salmonella enterica subsp.
enterica, thus representing a risk to human and animal health.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ramos, Carolina Pantuzza& Santana, Jordana Almeida& Morcatti Coura, Fernanda& Xavier, Rafael Gariglio Clark& Leal, Carlos Augusto Gomes& Oliveira Junior, Carlos Augusto…[et al.]. 2019. Identification and Characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile Isolates from Reptiles in Brazil. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128613
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ramos, Carolina Pantuzza…[et al.]. Identification and Characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile Isolates from Reptiles in Brazil. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1128613
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ramos, Carolina Pantuzza& Santana, Jordana Almeida& Morcatti Coura, Fernanda& Xavier, Rafael Gariglio Clark& Leal, Carlos Augusto Gomes& Oliveira Junior, Carlos Augusto…[et al.]. Identification and Characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile Isolates from Reptiles in Brazil. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128613
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128613
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر