Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma
المؤلفون المشاركون
Rave-Fränk, Margret
Ihler, Friedrich
Bertlich, Mattis
Weiss, Bernhard
Canis, Martin
Gratz, Ronja
Wolff, Hendrik A.
Kitz, Julia
Salinas, Gabriela
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-06
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In epithelial tumors, a shift towards a mesenchymal phenotype has been associated with increased invasiveness and metastasis.
It is assumed that this phenomenon plays a major role in disease progression and ultimately prognosis.
This study investigated epithelial-mesenchymal transition (EMT) in human papillomavirus- (HPV-) negative pharyngeal squamous cell carcinoma.
Tissue was obtained from one hypopharyngeal primary tumor and a regional lymph node metastasis during surgery with curative intention.
A cell culture was established from the primary tumor and mesenchymal growth conditions were emulated.
Gene expression profiling was performed (Human 8 × 60 K design array, Agilent Technologies) and EMT was assessed by a gene set (MSigDB: M5930, Hallmark_epithelial_mesenchymal_transition), applying gene set expression analysis (GSEA).
Immunohistochemical staining and flow cytometry of CD44 and E-cadherin were compared in primary tumor, metastasis, and cell cultures.
Primary tumor and metastasis were highly positive for CD44.
A loss of E-cadherin occurred in the metastasis.
Flow cytometry showed the appearance of a population without E-cadherin in spheroid colonies.
In GSEA, the EMT phenotype was enriched in the primary tumor compared to metastasis and cell cultures (FDR < 25%, p < 5%).
EMT showed variable expression during metastasis.
It may thereby be a dynamic state in HPV-negative pharyngeal squamous cell carcinoma that is active only during the process of metastasis itself.
Thereby, the primary tumor as well as the metastasis may exhibit fewer EMT properties.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ihler, Friedrich& Gratz, Ronja& Wolff, Hendrik A.& Weiss, Bernhard& Bertlich, Mattis& Kitz, Julia…[et al.]. 2018. Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128792
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ihler, Friedrich…[et al.]. Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1128792
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ihler, Friedrich& Gratz, Ronja& Wolff, Hendrik A.& Weiss, Bernhard& Bertlich, Mattis& Kitz, Julia…[et al.]. Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1128792
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128792
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر