Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate IschemiaReperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein
المؤلفون المشاركون
Chen, Yue
Tang, Xiaochen
Li, Ping
Zhou, Ying
Xue, Ting
Liu, Jie
Yu, Chen
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-11
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Aims.
To investigate whether bone marrow derived mesenchymal stromal cells (BMSC) have ameliorated ischemia/reperfusion injury-induced acute kidney injury (IRI-AKI) via tumor necrosis factor-inducible gene 6 protein (TSG-6) and how TSG-6 exerted this effect.
Methods.
We used lentiviral vectors of short hairpin RNA (shRNA) targeting TSG-6 gene to silence TSG-6 in BMSC.
And TSG-6-silenced BMSC were administrated into IRI-AKI rats.
Then we analyzed serum creatinine (Scr) and renal histology of IRI-AKI rats treated with BMSC after different pretreatments.
Furthermore, we explored the effect of TSG-6 on renal tubular epithelial cells proliferation in vivo and in vitro assays.
Results.
The Scr levels of IRI-AKI rats treated with BMSC (73.5±7.8 μmol/L) significantly decreased compared to those of IRI-AKI rats treated without BMSC (392.5±24.8 μmol/L, P<0.05) or with DMEM (314.0±19.8 μmol/L, P<0.05).
Meanwhile, the renal tissue injury in IRI-AKI rats treated with BMSC improved markedly.
However, the Scr levels of IRI-AKI rats treated with TSG-6-silenced BMSC (265.1±21.2 μmol/L) significantly increased compared to those with BMSC (74.0±8.5 μmol/L, P<0.05).
The proportion of Ki67-positive cells was reduced in IRI-AKI rats treated with TSG-6-silenced BMSC compared to that in IRI-AKI rats treated with BMSC (29.7±0.8% versus 43.4±3.0%, P<0.05).
In vitro, the cell proliferation rate of TSG-6-stimulated NRK-52E cells under hypoxia (89.2±3.9%) increased significantly compared to that of NRK-52E cells alone under hypoxia (82.4±0.8%, P<0.05).
Similarly, the proportion of Ki67-positive cells was significantly elevated in TSG-6-stimulated NRK-52E cells under hypoxia.
Furthermore, TSG-6 could inhibit infiltration of neutrophils in kidney tissue of IRI-AKI.
Conclusions.
TSG-6 plays a key role in the treatment of IRI-AKI with BMSC, which may be due to its effect on promoting renal tubular epithelial cells proliferation by modulating inflammation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Yue& Tang, Xiaochen& Li, Ping& Zhou, Ying& Xue, Ting& Liu, Jie…[et al.]. 2019. Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate IschemiaReperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1128877
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Yue…[et al.]. Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate IschemiaReperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1128877
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Yue& Tang, Xiaochen& Li, Ping& Zhou, Ying& Xue, Ting& Liu, Jie…[et al.]. Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate IschemiaReperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1128877
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1128877
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر