Deficiency of Dietary Fiber in Slc5a8-Null Mice Promotes Bacterial Dysbiosis and Alters Colonic Epithelial Transcriptome towards Proinflammatory Milieu
المؤلفون المشاركون
Sivaprakasam, Sathish
Ganapathy, Pramodh K.
Sikder, Mohd Omar Faruk
Elmassry, Moamen
Ramachandran, Sabarish
Kottapalli, Kameswara Rao
Ganapathy, Vadivel
المصدر
Canadian Journal of Gastroenterology and Hepatology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-28
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Inflammatory bowel disease (IBD) is characterized by chronic inflammation in the intestinal tract due to disruption of the symbiotic relationship between the host immune system and microbiota.
Various factors alter the gut microbiota which lead to dysbiosis; in particular, diet and dietary fibers constitute important determinants.
Dietary fiber protects against IBD; bacteria ferment these dietary fibers in colon and generate short-chain fatty acids (SCFAs), which mediate the anti-inflammatory actions of dietary fibers.
SLC5A8 is a high-affinity transporter in the apical membrane of colonic epithelium which mediates the entry of SCFAs from the lumen into cells in Na+-coupled manner.
Due to the unique transport kinetics, the function of the transporter becomes important only under conditions of low dietary fiber intake.
Here, we have examined the impact of dietary fiber deficiency on luminal microbial composition and transcriptomic profile in colonic epithelium in wild-type (WT) and Slc5a8-null (KO) mice.
We fed WT and KO mice with fiber-containing diet (FC-diet) or fiber-free diet (FF-diet) and analyzed the luminal bacterial composition by sequencing 16S rRNA gene in feces.
Interestingly, results showed significant differences in the microbial community depending on dietary fiber content and on the presence or absence of Slc5a8.
There were also marked differences in the transcriptomic profile of the colonic epithelium depending on the dietary fiber content and on the presence or absence of Slc5a8.
We conclude that absence of fiber in diet in KO mice causes bacterial dysbiosis and alters gene expression in the colon that is conducive for inflammation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sivaprakasam, Sathish& Ganapathy, Pramodh K.& Sikder, Mohd Omar Faruk& Elmassry, Moamen& Ramachandran, Sabarish& Kottapalli, Kameswara Rao…[et al.]. 2019. Deficiency of Dietary Fiber in Slc5a8-Null Mice Promotes Bacterial Dysbiosis and Alters Colonic Epithelial Transcriptome towards Proinflammatory Milieu. Canadian Journal of Gastroenterology and Hepatology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1129792
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sivaprakasam, Sathish…[et al.]. Deficiency of Dietary Fiber in Slc5a8-Null Mice Promotes Bacterial Dysbiosis and Alters Colonic Epithelial Transcriptome towards Proinflammatory Milieu. Canadian Journal of Gastroenterology and Hepatology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1129792
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sivaprakasam, Sathish& Ganapathy, Pramodh K.& Sikder, Mohd Omar Faruk& Elmassry, Moamen& Ramachandran, Sabarish& Kottapalli, Kameswara Rao…[et al.]. Deficiency of Dietary Fiber in Slc5a8-Null Mice Promotes Bacterial Dysbiosis and Alters Colonic Epithelial Transcriptome towards Proinflammatory Milieu. Canadian Journal of Gastroenterology and Hepatology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1129792
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1129792
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر