Integration of Gene Expression Profile Data to Screen and Verify Hub Genes Involved in Osteoarthritis
المؤلفون المشاركون
Du, Zhenwu
Yang, Qiwei
Wang, Qingyu
Zhang, Guizhen
Chen, Gaoyang
Li, Zhaoyan
Song, Yang
Li, Xin
Ren, Ming
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Osteoarthritis (OA) is one of the most common diseases worldwide, but the pathogenic genes and pathways are largely unclear.
The aim of this study was to screen and verify hub genes involved in OA and explore potential molecular mechanisms.
The expression profiles of GSE12021 and GSE55235 were downloaded from the Gene Expression Omnibus (GEO) database, which contained 39 samples, including 20 osteoarthritis synovial membranes and 19 matched normal synovial membranes.
The raw data were integrated to obtain differentially expressed genes (DEGs) and were deeply analyzed by bioinformatics methods.
The Gene Ontology (GO) and pathway enrichment of DEGs were performed by DAVID and Kyoto Encyclopedia of Genes and Genomes (KEGG) online analyses, respectively.
The protein-protein interaction (PPI) networks of the DEGs were constructed based on data from the STRING database.
The top 10 hub genes VEGFA, IL6, JUN, IL1β, MYC, IL4, PTGS2, ATF3, EGR1, and DUSP1 were identified from the PPI network.
Module analysis revealed that OA was associated with significant pathways including TNF signaling pathway, cytokine-cytokine receptor interaction, and osteoclast differentiation.
The qRT-PCR result showed that the expression level of IL6, VEGFA, JUN, IL-1β, and ATF3 was significantly increased in OA samples (p < 0.05), and these candidate genes could be used as potential diagnostic biomarkers and therapeutic targets of OA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Zhaoyan& Wang, Qingyu& Chen, Gaoyang& Li, Xin& Yang, Qiwei& Du, Zhenwu…[et al.]. 2018. Integration of Gene Expression Profile Data to Screen and Verify Hub Genes Involved in Osteoarthritis. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129793
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Zhaoyan…[et al.]. Integration of Gene Expression Profile Data to Screen and Verify Hub Genes Involved in Osteoarthritis. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129793
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Zhaoyan& Wang, Qingyu& Chen, Gaoyang& Li, Xin& Yang, Qiwei& Du, Zhenwu…[et al.]. Integration of Gene Expression Profile Data to Screen and Verify Hub Genes Involved in Osteoarthritis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129793
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1129793
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر