Molecular Detection of Bartonella spp. in China and St. Kitts
المؤلفون المشاركون
Zhang, Jilei
Kelly, Patrick
Wang, Chengming
Yang, Yi
Huang, Ke
Liu, Weiguo
Kalalah, Anwar
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-03
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Bartonella are vector-borne hemotropic bacteria that infect a wide variety of hosts, including people.
While there are PCR assays that can identify individual or groups of Bartonella, there is no reliable molecular method to simultaneously detect all species while maintaining genus specificity and sensitivity.
By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp.
on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR.
Then, a gltA-based Bartonella PCR was established by selecting primers for a highly variable region of gltA, of which the sequenced amplicons could identify individual Bartonella spp.
The pan-Bartonella FRET-qPCR did not detect negative controls (Brucella spp., Anaplasma spp., Rickettsia spp., Coxiella burnetii, and Wolbachia) but reliably detected as few as two copies of the positive control (Bartonella henselae) per reaction.
There was complete agreement between the pan-Bartonella FRET-qPCR and the gltA-based Bartonella PCR in detecting Bartonella in convenience test samples from China and St.
Kitts: cats (26%; 81/310), Ctenocephalides felis (20%; 12/60), cattle (24%; 23/98), and donkeys (4%; 1/20).
Sequencing of the gltA-based Bartonella PCR products revealed B.
henselae (70%; 57/81) and B.
clarridgeiae (30%; 24/81) in cats and C.
felis (67%; 8/12, and 33%; 4/12, respectively) and B.
bovis in cattle (23.5%; 23/98) and donkeys (4.0%; 1/24).
The pan-Bartonella FRET-qPCR and gltA-based Bartonella PCR we developed are highly sensitive and specific in detecting recognized Bartonella spp.
in a single reaction.
The pan-Bartonella FRET-qPCR is convenient requiring no gel electrophoresis and providing copy numbers, while the gltA-based Bartonella PCR reliably differentiates individual Bartonella species.
The use of these PCRs should greatly facilitate large-scale surveillance studies and the diagnosis of infections in clinical samples.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Ke& Kelly, Patrick & Zhang, Jilei& Yang, Yi& Liu, Weiguo& Kalalah, Anwar…[et al.]. 2019. Molecular Detection of Bartonella spp. in China and St. Kitts. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1130041
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Ke…[et al.]. Molecular Detection of Bartonella spp. in China and St. Kitts. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1130041
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Ke& Kelly, Patrick & Zhang, Jilei& Yang, Yi& Liu, Weiguo& Kalalah, Anwar…[et al.]. Molecular Detection of Bartonella spp. in China and St. Kitts. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1130041
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130041
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر