Antibacterial and Antibiofilm Activities of Nonpolar Extracts of Allium stipitatum Regel. against Multidrug Resistant Bacteria
المؤلفون المشاركون
van Belkum, Alex
Neela, Vasantha Kumari
Chigurupati, Sridevi
Karunanidhi, Arunkumar
Pichika, Mallikarjuna Rao
Lung, Leslie Than Thian
Mohd Fauzi, Fazlin
Ghaznavi-Rad, Ehsanollah
Hamat, Rukman Awang
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The present study assessed the in vitro antibacterial and antibiofilm potential of hexane (ASHE) and dichloromethane (ASDE) extracts of Allium stipitatum (Persian shallot) against planktonic cells and biofilm structures of clinically significant antibiotic resistant pathogens, with a special emphasis on methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant S.
aureus (MRSA), and emerging pathogens, Acinetobacter baumannii and Stenotrophomonas maltophilia.
Antibacterial activities were determined through disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill kinetics, and electron microscopy.
Antibiofilm activity was assessed by XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] reduction assay and by confocal laser scanning microscopy (CLSM).
The zone of inhibition ranged from 13 to 33 mm, while the MICs and MBCs ranged from 16 to 1024 μg mL−1.
Both ASHE and ASDE completely eradicated overnight cultures of the test microorganisms, including antibiotic resistant strains.
Time-kill studies showed that the extracts were strongly bactericidal against planktonic cultures of S.
aureus, MRSA, Acinetobacter baumannii, and S.
maltophilia as early as 4 hours postinoculation (hpi).
ASHE and ASDE were shown to inhibit preformed biofilms of the four biofilm phenotypes tested.
Our results demonstrate the potential therapeutic application of ASHE and ASDE to inhibit the growth of gram-positive and gram-negative biofilms of clinical significance and warrant further investigation of the potential of A.
stipitatum bulbs against biofilm-related drug resistance.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Karunanidhi, Arunkumar& Ghaznavi-Rad, Ehsanollah& Hamat, Rukman Awang& Pichika, Mallikarjuna Rao& Lung, Leslie Than Thian& Mohd Fauzi, Fazlin…[et al.]. 2018. Antibacterial and Antibiofilm Activities of Nonpolar Extracts of Allium stipitatum Regel. against Multidrug Resistant Bacteria. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1130077
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Karunanidhi, Arunkumar…[et al.]. Antibacterial and Antibiofilm Activities of Nonpolar Extracts of Allium stipitatum Regel. against Multidrug Resistant Bacteria. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1130077
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Karunanidhi, Arunkumar& Ghaznavi-Rad, Ehsanollah& Hamat, Rukman Awang& Pichika, Mallikarjuna Rao& Lung, Leslie Than Thian& Mohd Fauzi, Fazlin…[et al.]. Antibacterial and Antibiofilm Activities of Nonpolar Extracts of Allium stipitatum Regel. against Multidrug Resistant Bacteria. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1130077
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130077
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر