Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis
المؤلفون المشاركون
Rizzo, Giuseppina
Lo Giudice, Roberto
Lo Giudice, G.
Cervino, G.
Centofanti, A.
Favaloro, A.
Rizzo, D.
Squeri, R.
Costa, B. G.
La Fauci, V.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Introduction.
The use of equine bone blocks is widely reported for bone augmentation techniques.
The block must be shaped according to the form of the defect that should be regenerated.
The shaping could be performed by hand before or during the surgery, in a sterile ambient, or using a CNC milling machine that could not be sterile.
The aim of our study was to evaluate if a steam sterilization could provide a medical grade sterilization of the blocks and to evaluate if bone microstructure and collagen structures change after different steam sterilization protocols provided by mainstream autoclave.
Materials and Method.
Two blocks of equine bone were divided into 16 samples.
1 sample was used as control and not submitted to any treatment.
15 samples were infected with a Streptococcus faecalis bacterial culture.
The samples were singularly packed, randomly divided into 3 groups, and submitted to autoclave sterilization on the same device.
The groups were submitted to a sterilization cycle (Gr.
A: 121°C, 1,16 bar for 20′; Gr.
B:134°C, 2,16 bar for 4′; Gr.
C: 134°C, 2,16 bar for 3.30 min.).
2 samples for each group were evaluated for the sterility.
3 samples for each group were observed at SEM to notice the macro- and microstructure modification and to confocal microscope to observe the collagen.
Results.
All samples were sterile.
The SEM evaluation showed, in all groups, a preserved morphological structure.
Confocal microscope evaluation shows that the collagen structure appears to be more uniform and preserved in group C.
Conclusion.
Data show that autoclave steam sterilization could be reliable to obtain sterilization of equine bone blocks.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lo Giudice, Roberto& Rizzo, Giuseppina& Centofanti, A.& Favaloro, A.& Rizzo, D.& Cervino, G.…[et al.]. 2018. Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130097
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lo Giudice, Roberto…[et al.]. Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1130097
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lo Giudice, Roberto& Rizzo, Giuseppina& Centofanti, A.& Favaloro, A.& Rizzo, D.& Cervino, G.…[et al.]. Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130097
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130097
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر