Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley)
المؤلفون المشاركون
Hu, Jialiang
Wu, Yue
Xie, Huifang
Shi, Wanyin
Chen, Zhiyuan
Jiang, Dan
Hu, Hui
Zheng, Xiangwei
Xu, Jian
Yang, Yuejun
Liu, Yuancai
المصدر
Advances in Polymer Technology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background and Objective.
Qingke (Tibetan hulless barley, Hordeum vulgare L.) contains a high content of β-glucan among all the cereal varieties.
Although β-glucan has multiple physiological functions, the physiological function of qingke β-glucan was few studied.
In this study, the β-glucan was isolated, purified, determined the structural characterization, and measured the inhibitory activity to enzymes correlating blood sugar and lipid.
Methods.
β-Glucan was isolated from enzymatic aqueous extract of qingke by using deproteinization, decolorization, DEAE-52 column chromatography, and sepharose CL-4B agarose gel column chromatography.
The structure of the β-glucan was determined using FT-IR and 13C-NMR spectra analysis, and molecular mass by use of HPSEC-dRI-LS.
The kinematic viscosity was measured.
The inhibitory effects of this β-glucan on four enzymes were investigated.
Results.
This β-glucan had a uniform molecular weight of 201,000 Da with β-(1⟶4) as the main chain and β-(1⟶3) as a side chain.
The β-glucan presented a relatively strong inhibitory activity on α-glucosidase, moderate inhibition on invertase, and a weak inhibition on α-amylase, whereas it did not inhibit lipase.
Conclusion.
The study indicates that the enzymatic β-glucan from qingke has the potential as natural auxiliary hypoglycemic additives in functional medicine or foods.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Jialiang& Wu, Yue& Xie, Huifang& Shi, Wanyin& Chen, Zhiyuan& Jiang, Dan…[et al.]. 2020. Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley). Advances in Polymer Technology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130205
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Jialiang…[et al.]. Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley). Advances in Polymer Technology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1130205
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Jialiang& Wu, Yue& Xie, Huifang& Shi, Wanyin& Chen, Zhiyuan& Jiang, Dan…[et al.]. Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley). Advances in Polymer Technology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130205
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130205
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر