Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins
المؤلفون المشاركون
González-Valdez, José
Mejía-Manzano, Luis Alberto
Vázquez-Villegas, Patricia
المصدر
Advances in Polymer Technology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-25
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In recent years, the effective purification of PEGylated therapeutic proteins from reaction media has received particular attention.
Although several techniques have been used, affinity-based strategies have been scarcely explored despite the fact that, after PEGylation, marked changes in the molecular affinity parameters of the modified molecules are observed.
With this in mind, future contributions in the bioseparation of these polymer-protein conjugates are expected to exploit affinity in chromatographic and nonchromatographic techniques which will surely derive in the integration of different operations.
However, this will only occur as novel ligands which are simultaneously found.
As it will be mentioned, these novel ligands may be screened or designed.
In both cases, computer-aided tools will support their identification or development.
Additionally, ligand discovery by high-throughput screening (HTS) is believed to become a fast, economic, and informative technology that will aid in the mass production of ligands along with genetic engineering and related technologies.
Therefore, besides analyzing the state of the art in affinity separation strategies for PEGylated molecules, this review proposes a basic guideline for the selection of adequate ligands to provide information and prospective on the future of affinity operations in solving this particular bioengineering problem.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mejía-Manzano, Luis Alberto& Vázquez-Villegas, Patricia& González-Valdez, José. 2020. Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins. Advances in Polymer Technology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1130292
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mejía-Manzano, Luis Alberto…[et al.]. Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins. Advances in Polymer Technology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1130292
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mejía-Manzano, Luis Alberto& Vázquez-Villegas, Patricia& González-Valdez, José. Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins. Advances in Polymer Technology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1130292
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130292
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر