Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration
المؤلفون المشاركون
Heimel, Patrick
Swiadek, Nicole Victoria
Slezak, Paul
Kerbl, Markus
Schneider, Cornelia
Nürnberger, Sylvia
Redl, Heinz
Teuschl, Andreas Herbert
Hercher, David
المصدر
Contrast Media & Molecular Imaging
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-27
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Microcomputed tomography (μCT) is widely used for the study of mineralized tissues, but a similar use for soft tissues is hindered by their low X-ray attenuation.
This limitation can be overcome by the recent development of different staining techniques.
Staining with Lugol’s solution, a mixture of one part iodine and two parts potassium iodide in water, stands out among these techniques for its low complexity and cost.
Currently, Lugol staining is mostly used for anatomical examination of tissues.
In the present study, we seek to optimize the quality and reproducibility of the staining for ex vivo visualization of soft tissues in the context of a peripheral nerve regeneration model in the rat.
We show that the staining result not only depends on the concentration of the staining solution but also on the amount of stain in relation to the tissue volume and composition, necessitating careful adaptation of the staining protocol to the respective specimen tissue.
This optimization can be simplified by a stepwise staining which we show to yield a similar result compared to staining in a single step.
Lugol staining solution results in concentration-dependent tissue shrinkage which can be minimized but not eliminated.
We compared the shrinkage of tendon, nerve, skeletal muscle, heart, brain, and kidney with six iterations of Lugol staining.
60 ml of 0.3% Lugol’s solution per cm3 of tissue for 24 h yielded good results on the example of a peripheral nerve regeneration model, and we were able to show that the regenerating nerve inside a silk fibroin tube can be visualized in 3D using this staining technique.
This information helps in deciding the region of interest for histological imaging and provides a 3D context to histological findings.
Correlating both imaging modalities has the potential to improve the understanding of the regenerative process.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Heimel, Patrick& Swiadek, Nicole Victoria& Slezak, Paul& Kerbl, Markus& Schneider, Cornelia& Nürnberger, Sylvia…[et al.]. 2019. Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration. Contrast Media & Molecular Imaging،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1130349
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Heimel, Patrick…[et al.]. Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration. Contrast Media & Molecular Imaging No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1130349
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Heimel, Patrick& Swiadek, Nicole Victoria& Slezak, Paul& Kerbl, Markus& Schneider, Cornelia& Nürnberger, Sylvia…[et al.]. Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration. Contrast Media & Molecular Imaging. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1130349
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130349
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر