Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells
المؤلفون المشاركون
Aalami, Amir Hossein
Mesgari, Mohammad
Sahebkar, Amirhossein
المصدر
Bioinorganic Chemistry and Applications
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-20
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Changes in the expression of microRNAs can affect cancer cells’ viability and behavior and the impact on cancer treatment.
In this study, the expression of miR-155-5p, miR-203a-3p, and miR-223-3p in the MCF7 cancer cell line was studied when exposed to ZnO nanoparticles synthesized through a green route.
Mentioned ZnO-NPs were well characterized by UV-vis spectroscopy, DLS, XRD, FTIR, FE-SEM, EDX, zeta potential, and AFM analyses.
Cellular studies were conducted using ZnO-NPs before miRNA investigations including MTT cytotoxicity test against MCF7, MDA-MB-231, and HFF cell lines.
Moreover, apoptosis assays were performed using morphological analysis, fluorescent dyes, flow cytometry, and evaluation of caspase-3 and caspase-8 gene expression.
Biological properties such as the antioxidant and antimicrobial activity of these novel ZnO-NPs were considered.
MTT assays showed that the inhibitory concentration (IC50) of ZnO-NPs after 24 h was 11.16 μg/mL, 60.08 μg/mL, and 26.3 μg/mL on MCF7, MDA-MB-231, and HFF cells, respectively.
The qRT-PCR results showed reduced expression of miR-155-5p, miR-203a-3p, and miR-223-3p when the MCF7 cells were treated with the IC50 concentration of ZnO-NPs (11.16 μg/mL).
The antioxidant activity results showed EC50 values at 57.19 μg/mL and 31.5 μg/mL in DPPH and ABTS assays, respectively.
The antimicrobial activity of ZnO-NPs was determined on Gram-negative and Gram-positive bacterial strains and fungi using MIC and MBC assays.
These NPs had a significant effect in reducing the expression of microRNAs in breast cancer cells.
Finally, ZnO-NPs exerted antioxidant and antimicrobial activities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Aalami, Amir Hossein& Mesgari, Mohammad& Sahebkar, Amirhossein. 2020. Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells. Bioinorganic Chemistry and Applications،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1130732
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Aalami, Amir Hossein…[et al.]. Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells. Bioinorganic Chemistry and Applications No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1130732
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Aalami, Amir Hossein& Mesgari, Mohammad& Sahebkar, Amirhossein. Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells. Bioinorganic Chemistry and Applications. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1130732
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130732
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر