Continuous Readout versus Titer-Based Assays of Influenza Vaccine Trials: Sensitivity, Specificity, and False Discovery Rates
المؤلفون المشاركون
Li, Dongmei
Wang, Jiong
Treanor, John J.
Zand, Martin S.
Garigen, Jessica
المصدر
Computational and Mathematical Methods in Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The current gold standard for measuring antibody-based immunity to influenza viruses relies on the hemagglutinin inhibition assay (HAI), an 80-year-old technology, and the microneutralization assay (MN).
Both assays use serial dilution to provide a discrete, ranked readout of 8–14 categorical titer values for each sample.
In contrast to other methods of measuring vaccine antibody levels that produce a continuous readout (i.e., mPLEX-Flu and ELISA), titering methods introduce imprecision and increase false discovery rates (FDR).
In this paper, we assess the degree of such statistical errors, first with simulation studies comparing continuous data with titer data in influenza vaccine study group comparison analyses and then by analyzing actual sample data from an influenza vaccine trial.
Our results show the superiority of using continuous, rather than discrete, readout assays.
Compared to continuous readout assays, titering assays have a lower statistical precision and a higher FDR.
The results suggested that traditional titering assays could lead to increased Type-II errors in the comparison of different therapeutic arms of an influenza vaccine trial.
These statistical issues are related to the mathematical nature of titer-based assays, which we examine in detail in the simulation studies.
Continuous readout assays are free of this issue, and thus it is possible that comparisons of study groups could provide different results with these two methods as we have shown in our case study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Dongmei& Wang, Jiong& Garigen, Jessica& Treanor, John J.& Zand, Martin S.. 2019. Continuous Readout versus Titer-Based Assays of Influenza Vaccine Trials: Sensitivity, Specificity, and False Discovery Rates. Computational and Mathematical Methods in Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1130780
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Dongmei…[et al.]. Continuous Readout versus Titer-Based Assays of Influenza Vaccine Trials: Sensitivity, Specificity, and False Discovery Rates. Computational and Mathematical Methods in Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1130780
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Dongmei& Wang, Jiong& Garigen, Jessica& Treanor, John J.& Zand, Martin S.. Continuous Readout versus Titer-Based Assays of Influenza Vaccine Trials: Sensitivity, Specificity, and False Discovery Rates. Computational and Mathematical Methods in Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1130780
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130780
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر