Transcriptome Analysis of Porcine PBMCs Reveals the Immune Cascade Response and Gene Ontology Terms Related to Cell Death and Fibrosis in the Progression of Liver Failure
المؤلفون المشاركون
Zhang, YiMin
Shao, Li
Zhou, Ning
Li, JianZhou
Chen, Yu
Lu, Juan
Wang, Jie
Chen, ErMei
Xie, ZhongYang
Li, Lanjuan
المصدر
Canadian Journal of Gastroenterology and Hepatology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
The key gene sets involved in the progression of acute liver failure (ALF), which has a high mortality rate, remain unclear.
This study aims to gain a deeper understanding of the transcriptional response of peripheral blood mononuclear cells (PBMCs) following ALF.
Methods.
ALF was induced by D-galactosamine (D-gal) in a porcine model.
PBMCs were separated at time zero (baseline group), 36 h (failure group), and 60 h (dying group) after D-gal injection.
Transcriptional profiling was performed using RNA sequencing and analysed using DAVID bioinformatics resources.
Results.
Compared with the baseline group, 816 and 1,845 differentially expressed genes (DEGs) were identified in the failure and dying groups, respectively.
A total of five and two gene ontology (GO) term clusters were enriched in 107 GO terms in the failure group and 154 GO terms in the dying group.
These GO clusters were primarily immune-related, including genes regulating the inflammasome complex and toll-like receptor signalling pathways.
Specifically, GO terms related to cell death, including apoptosis, pyroptosis, and autophagy, and those related to fibrosis, coagulation dysfunction, and hepatic encephalopathy were enriched.
Seven Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, cytokine-cytokine receptor interaction, hematopoietic cell lineage, lysosome, rheumatoid arthritis, malaria, and phagosome and pertussis pathways were mapped for DEGs in the failure group.
All of these seven KEGG pathways were involved in the 19 KEGG pathways mapped in the dying group.
Conclusion.
We found that the dramatic PBMC transcriptome changes triggered by ALF progression was predominantly related to immune responses.
The enriched GO terms related to cell death, fibrosis, and so on, as indicated by PBMC transcriptome analysis, seem to be useful in elucidating potential key gene sets in the progression of ALF.
A better understanding of these gene sets might be of preventive or therapeutic interest.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, YiMin& Shao, Li& Zhou, Ning& Li, JianZhou& Chen, Yu& Lu, Juan…[et al.]. 2018. Transcriptome Analysis of Porcine PBMCs Reveals the Immune Cascade Response and Gene Ontology Terms Related to Cell Death and Fibrosis in the Progression of Liver Failure. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130884
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, YiMin…[et al.]. Transcriptome Analysis of Porcine PBMCs Reveals the Immune Cascade Response and Gene Ontology Terms Related to Cell Death and Fibrosis in the Progression of Liver Failure. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1130884
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, YiMin& Shao, Li& Zhou, Ning& Li, JianZhou& Chen, Yu& Lu, Juan…[et al.]. Transcriptome Analysis of Porcine PBMCs Reveals the Immune Cascade Response and Gene Ontology Terms Related to Cell Death and Fibrosis in the Progression of Liver Failure. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130884
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130884
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر