Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study
المؤلفون المشاركون
Wang, Yun-Cang
Wang, Zhi-Qiang
Yuan, Yong
Ren, Tao
Ni, Peng-Zhi
Chen, Long-Qi
المصدر
Canadian Journal of Gastroenterology and Hepatology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-26
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
To explore the role of Notch signaling in the development of Barrett’s esophagus.
Methods.
Patients with esophagectomy and gastric interposition were recruited as a human model of gastroesophageal reflux disease.
The expressions of Notch signaling genes in normal esophagus from surgical specimen and columnar metaplasia in the esophageal remnant after esophagectomy were evaluated by real time quantitative Polymerase Chain Reaction (RT-qPCR) and immunohistochemistry (IHC).
For in vitro experiments, Het-1A cells were treated with hydrochloric acid, deoxycholic acid, mixture of hydrochloric acid and deoxycholic acid, or Notch1-siRNA, and expressions of Notch1, Hes1, MUC2, and K13 were evaluated via RT-qPCR and western blot.
Results.
Samples were obtained from 36 patients with columnar metaplasia in the esophageal remnant.
Both IHC and RT-qPCR indicated that Notch1 and Hes1 expressions were significantly higher in normal esophagus than that in metaplasia.
Hydrochloric acid and deoxycholic acid suppressed Notch1, Hes1, and K13 expressions, in concert with increasing MUC2 expressions.
Notch inhibition by Notch1-siRNA contributed to the downregulation of Notch1, Hes1, and K13 expressions, whereas MUC2 expression was enhanced.
Conclusions.
Both hydrochloric acid and deoxycholic acid could suppress Notch signaling pathway in esophageal epithelial cells, and inhibited Notch signaling has important functions in the development of Barrett’s esophagus.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yun-Cang& Wang, Zhi-Qiang& Yuan, Yong& Ren, Tao& Ni, Peng-Zhi& Chen, Long-Qi. 2018. Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1130908
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yun-Cang…[et al.]. Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1130908
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yun-Cang& Wang, Zhi-Qiang& Yuan, Yong& Ren, Tao& Ni, Peng-Zhi& Chen, Long-Qi. Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1130908
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130908
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر