Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MSMS and Its Application in Pharmacokinetics and Bioavailability
المؤلفون المشاركون
Zhang, Meiling
Chen, Lian-Guo
Ma, Jianshe
Wang, Xianqin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-17
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
A specific ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination of six Uncaria alkaloids in mouse blood with midazolam as the internal standard (IS).
Only 20 μL blood was needed for sample preparation, and the protein was precipitated with acetonitrile.
The UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) was used for chromatographic separation.
The mobile phase consisted of 0.1% formic acid and acetonitrile with gradient elution within 5.5 min.
Multiple reaction monitoring (MRM) and the positive electrospray ionization model were used for quantitative analysis.
The accuracy of the UPLC-MS/MS method ranged from 86.5% to 110.4%.
The precision for intraday and interday was ≤15% each.
The mean recovery and the matrix effects were found to be 64.4-86.8% and 94.1-109.4%, respectively.
The calibration curves in blood were linear in the range of 1-1000 ng/mL with a favorable correlation coefficient (r2) of 0.995.
The pharmacokinetic results showed that six Uncaria alkaloids metabolized rapidly in mice with a half-life between 0.6 h and 4.4 h.
The bioavailability of corynoxeine, isocorynoxeine, rhynchophylline, isorhynchophylline, hirsutine, and hirsuteine was 27.3%, 32.7%, 49.4%, 29.5%, 68.9%, and 51.0%, respectively, which showed satisfactory oral absorption of each alkaloid.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Lian-Guo& Ma, Jianshe& Wang, Xianqin& Zhang, Meiling. 2020. Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MSMS and Its Application in Pharmacokinetics and Bioavailability. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1131388
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Lian-Guo…[et al.]. Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MSMS and Its Application in Pharmacokinetics and Bioavailability. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1131388
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Lian-Guo& Ma, Jianshe& Wang, Xianqin& Zhang, Meiling. Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MSMS and Its Application in Pharmacokinetics and Bioavailability. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1131388
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131388
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر