Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer
المؤلفون المشاركون
Khan, Khushbukhat
Safi, Sadia
Abbas, Asma
Badshah, Yasmin
Dilshad, Erum
Rafiq, Mehak
Zahra, Kainat
Shabbir, Maria
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-28
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Breast cancer is the most prevailing disease among women.
It actually develops from breast tissue and has heterogeneous and complex nature that constitutes multiple tumor quiddities.
These features are associated with different histological forms, distinctive biological characteristics, and clinical patterns.
The predisposition of breast cancer has been attributed to a number of genetic factors, associated with the worst outcomes.
Unfortunately, their behavior with relevance to clinical significance remained poorly understood.
So, there is a need to further explore the nature of the disease at the transcriptome level.
The focus of this study was to explore the influence of Krüppel-like factor 3 (KLF3), tumor protein D52 (TPD52), microRNA 124 (miR-124), and protein kinase C epsilon (PKCε) expression on breast cancer.
Moreover, this study was also aimed at predicting the tertiary structure of KLF3 protein.
Expression of genes was analyzed through real-time PCR using the delta cycle threshold method, and statistical significance was calculated by two-way ANOVA in Graphpad Prism.
For the construction of a 3D model, various bioinformatics software programs, Swiss Model and UCSF Chimera, were employed.
The expression of KLF3, miR-124, and PKCε genes was decreased (fold change: 0.076443, 0.06969, and 0.011597, respectively).
However, there was 2-fold increased expression of TPD52 with p value < 0.001 relative to control.
Tertiary structure of KLF3 exhibited 80.72% structure conservation with its template KLF4 and was 95.06% structurally favored by a Ramachandran plot.
These genes might be predictors of stage, metastasis, receptor, and treatment status and used as new biomarkers for breast cancer diagnosis.
However, extensive investigations at the tissue level and in in vivo are required to further strengthen their role as a potential biomarker for prognosis of breast cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khan, Khushbukhat& Safi, Sadia& Abbas, Asma& Badshah, Yasmin& Dilshad, Erum& Rafiq, Mehak…[et al.]. 2020. Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1131582
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khan, Khushbukhat…[et al.]. Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1131582
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khan, Khushbukhat& Safi, Sadia& Abbas, Asma& Badshah, Yasmin& Dilshad, Erum& Rafiq, Mehak…[et al.]. Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1131582
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131582
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر