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Mutations in the BRAF, NRAS, and C-KIT Genes of Patients Diagnosed with Melanoma in Colombia Population
المؤلفون المشاركون
Gutiérrez-Castañeda, Luz D.
Gamboa, Mauricio
Nova, John A.
Pulido, Leonardo
Tovar-Parra, Jose D.
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Introduction.
Mutations in the BRAF, NRAS, and C-KIT genes have been associated with the histopathological characteristics of melanoma.
Likewise, the incidence of each of these subtypes changes according to the geographical origin of the population analyzed.
Objective.
To determine the mutation frequency in exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C-KIT gene and to relate it with histological subtypes in patients from a region with high levels of ultraviolet radiation.
Methodology.
The clinicopathological characteristics of 54 cutaneous melanoma samples were analyzed.
Mutation analysis was performed via qPCR on paraffin-embedded tumor tissue samples using probes specific for the V600E mutation.
Amplification of exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C-KIT gene was performed for subsequent sequencing using the Sanger method.
Result.
The most frequent histological subtype in the analyzed sample was lentigo maligna/lentigo maligna melanoma (52%) followed by acral lentiginous melanoma (20%).
The BRAF-V600 variant was the most frequent (63.6%).
The most frequent (54%) mutation in NRAS was p.Lys5∗.
In the C-KIT gene, only the Val560Ala mutation was found.
Conclusion.
Differences in histological subtypes and mutations in the BRAF, NRAS, and C-KIT genes were found in the analyzed population.
This indicates that environmental and genetic factors significantly influence the introduction of the disease in this geographic region.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gutiérrez-Castañeda, Luz D.& Gamboa, Mauricio& Nova, John A.& Pulido, Leonardo& Tovar-Parra, Jose D.. 2020. Mutations in the BRAF, NRAS, and C-KIT Genes of Patients Diagnosed with Melanoma in Colombia Population. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132254
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gutiérrez-Castañeda, Luz D.…[et al.]. Mutations in the BRAF, NRAS, and C-KIT Genes of Patients Diagnosed with Melanoma in Colombia Population. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1132254
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gutiérrez-Castañeda, Luz D.& Gamboa, Mauricio& Nova, John A.& Pulido, Leonardo& Tovar-Parra, Jose D.. Mutations in the BRAF, NRAS, and C-KIT Genes of Patients Diagnosed with Melanoma in Colombia Population. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132254
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132254
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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