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High-Throughput Sequencing-Based Identification of Serum Exosomal Differential miRNAs in High-Grade Glioma and Intracranial Lymphoma
المؤلفون المشاركون
Wang, Shun
Xu, Zhenkuan
Zhang, Chao
Yu, Rui
Jiang, Jun
Wang, Chengwei
Qu, Chuncheng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
At present, no effective noninvasive method is currently available for the differential diagnosis of high-grade glioma and intracranial lymphoma.
In the present study, we aimed to screen microRNA (miRNA) markers in serum exosomes for differential diagnosis of high-grade glioma and intracranial lymphoma using high-throughput sequencing technology.
Methods.
Patients with intracranial lymphoma or high-grade glioma and healthy controls were included in this study (training cohort (n=10) and validation cohort: intracranial lymphoma (n=10), high-grade glioma (n=32), and healthy controls (n=20)).
After RNA was extracted from serum exosomes, the high-throughput sequencing was used to determine the expression profiles of serum exosomal miRNAs and screen the differentially expressed miRNAs.
RT-qPCR was used to verify the expressions of the selected miRNAs.
The differences of miRNA expressions between groups were assessed by the Kruskal-Wallis test.
The diagnostic value was analyzed using the receiver operating characteristic (ROC) curve.
Results.
High-throughput sequencing demonstrated that 170 miRNAs, including 109 upregulated ones and 61 downregulated ones, were differentially expressed in serum exosomes between the patients with intracranial lymphoma and high-grade glioma.
Compared with the healthy controls, the number of differential serum exosomal miRNAs in the high-grade glioma group and intracranial lymphoma group was 130 and 173, respectively.
RT-qPCR proved that both miR-766-5p and miR-376b-5p were significantly downregulated in high-grade glioma and intracranial lymphoma patients compared with the healthy controls (all p<0.001), and the expression of serum exosomal miR-766-5p in the intracranial lymphoma group was lower compared with the high-grade glioma group (p<0.05).
The areas under ROC curve (AUCs) of serum exosomal miR-766-5p and miR-376b-5p for the diagnosis of glioma were 0.8883 (p<0.001) and 0.7688 (p=0.001), respectively, and they were 0.9271 (p<0.001) and 0.8542 (p<0.001), respectively, for the diagnosis of intracranial lymphoma.
Moreover, the AUC value of serum exosomal miR-766-5p for the differential diagnosis of glioma and intracranial lymphoma was 0.7201 (p=0.026).
Conclusions.
miR-766-5p and miR-376b-5p in serum exosomes might be used as auxiliary diagnostic indicators for high-grade glioma and intracranial lymphoma, and miR-766-5p might be used as a differential diagnostic marker for both diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Shun& Xu, Zhenkuan& Zhang, Chao& Yu, Rui& Jiang, Jun& Wang, Chengwei…[et al.]. 2020. High-Throughput Sequencing-Based Identification of Serum Exosomal Differential miRNAs in High-Grade Glioma and Intracranial Lymphoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132327
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Shun…[et al.]. High-Throughput Sequencing-Based Identification of Serum Exosomal Differential miRNAs in High-Grade Glioma and Intracranial Lymphoma. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1132327
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Shun& Xu, Zhenkuan& Zhang, Chao& Yu, Rui& Jiang, Jun& Wang, Chengwei…[et al.]. High-Throughput Sequencing-Based Identification of Serum Exosomal Differential miRNAs in High-Grade Glioma and Intracranial Lymphoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132327
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132327
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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