Identification of Functional Genes in Pterygium Based on Bioinformatics Analysis
المؤلفون المشاركون
Lu, Chen
He, Siying
Dong, Shiqi
Qiao, Chen
Xu, Yuting
Wu, Xiying
Yan, Ming
Zheng, Fang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-23
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Purpose.
The competing endogenous RNA (ceRNA) network regulatory has been investigated in the occurrence and development of many diseases.
This research aimed at identifying the key RNAs of ceRNA network in pterygium and exploring the underlying molecular mechanism.
Methods.
Differentially expressed long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs were obtained from the Gene Expression Omnibus (GEO) database and analyzed with the R programming language.
LncRNA and miRNA expressions were extracted and pooled by the GEO database and compared with those in published literature.
The lncRNA-miRNA-mRNA network was constructed of selected lncRNAs, miRNAs, and mRNAs.
Metascape was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on mRNAs of the ceRNA network and to perform Protein-Protein Interaction (PPI) Network analysis on the String website to find candidate hub genes.
The Comparative Toxicogenomic Database (CTD) was used to find hub genes closely related to pterygium.
The differential expressions of hub genes were verified using the reverse transcription-real-time fluorescent quantitative PCR (RT-qPCR).
Result.
There were 8 lncRNAs, 12 miRNAs, and 94 mRNAs filtered to construct the primary ceRNA network.
A key lncRNA LIN00472 ranking the top 1 node degree was selected to reconstruct the LIN00472 network.
The GO and KEGG pathway enrichment showed the mRNAs in ceRNA networks mainly involved in homophilic cell adhesion via plasma membrane adhesion molecules, developmental growth, regulation of neuron projection development, cell maturation, synapse assembly, central nervous system neuron differentiation, and PID FOXM1 PATHWAY.
According to the Protein-Protein Interaction Network (PPI) analysis on mRNAs in LINC00472 network, 10 candidate hub genes were identified according to node degree ranking.
Using the CTD database, we identified 8 hub genes closely related to pterygium; RT-qPCR verified 6 of them were highly expressed in pterygium.
Conclusion.
Our research found LINC00472 might regulate 8 hub miRNAs (miR-29b-3p, miR-183-5p, miR-138-5p, miR-211-5p, miR-221-3p, miR-218-5p, miR-642a-5p, miR-5000-3p) and 6 hub genes (CDH2, MYC, CCNB1, RELN, ERBB4, RB1) in the ceRNA network through mainly PID FOXM1 PATHWAY and play an important role in the development of pterygium.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Yuting& Qiao, Chen& He, Siying& Lu, Chen& Dong, Shiqi& Wu, Xiying…[et al.]. 2020. Identification of Functional Genes in Pterygium Based on Bioinformatics Analysis. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132461
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Yuting…[et al.]. Identification of Functional Genes in Pterygium Based on Bioinformatics Analysis. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1132461
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Yuting& Qiao, Chen& He, Siying& Lu, Chen& Dong, Shiqi& Wu, Xiying…[et al.]. Identification of Functional Genes in Pterygium Based on Bioinformatics Analysis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132461
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132461
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر