USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1AKT Pathway
المؤلفون المشاركون
Lv, Dongqing
Chen, Meng
Xu, Hailing
Zhou, Suna
Yang, Haihua
Wang, Wei
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Previous studies have shown that ubiquitin-specific protease 46 (USP46) is a tumor suppressor in colon cancer and renal cell carcinoma.
However, its specific role in other cancers is still poorly understood.
This study is aimed at investigating the role of USP46 in lung cancer tumorigenesis and identifying its underlying mechanisms.
Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting (WB) were used to measure the expression levels of USP46 and PHLPP1 in lung cancer tissue and adjacent normal tissue from patients with lung cancer.
We examined the ability of USP46 to regulate cell proliferation in lung cancer cells via cell proliferation assay, radiation assay, genetic overexpression and knockdown, and chemical inhibition of relevant genes.
We investigated the underlying mechanisms in multiple lung cancer cell line models by coimmunoprecipitation and ubiquitination assays.
In this study, we identified a strong downregulation of the expressions of USP46 and PHLPP1 in lung cancer tissues relative to normal adjacent tissues.
USP46 was further shown to inhibit lung cancer cell proliferation under conditions of normal growth and during radiation-induced DNA damage by antagonizing the ubiquitination of PHLPP1 resulting in the inhibition of AKT signaling.
Exposure to radiation and AKT inhibition significantly reversed the effect of USP46 siRNA on lung cancer cell proliferation.
USP46 is downregulated in lung cancer and suppresses the proliferation of lung cancer cells by inhibiting the PHLPP1/AKT pathway.
AKT inhibition slows the proliferation of lung cancer cells that have been downregulated by USP46 and exposed to radiation.
This suggests a potential therapeutic avenue for USP46-downregulated lung cancer through a combination of radiation and AKT inhibitor treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Wei& Chen, Meng& Xu, Hailing& Lv, Dongqing& Zhou, Suna& Yang, Haihua. 2020. USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1AKT Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132560
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Wei…[et al.]. USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1AKT Pathway. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1132560
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Wei& Chen, Meng& Xu, Hailing& Lv, Dongqing& Zhou, Suna& Yang, Haihua. USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1AKT Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132560
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132560
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر