Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs
المؤلفون المشاركون
Alves, Carlos R.
Veloso, Laura Barral
de Oliveira Cardoso, Flávia
dos Santos Charret, Karen
de Sá Silva, Matheus Pereira
de Castro Côrtes, Luzia Monteiro
da Silva, Franklin Souza
de Sousa, Joel Fontes
Rocha, Marília Fonseca
Calabrese, Kátia da Silva
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Leishmania spp.
proteases have been proposed as virulence factors contributing to adaptive success these parasites to the mammalian hosts.
Since these enzymes are poorly studied in naturally infected dogs, this work aims to show the differences in metalloprotease and cysteine proteases gene expression in ear edge skin of dogs naturally infected by Leishmania (Leishmania) infantum.
A cohort of dogs (n=20) naturally infected by L.
(L.) infantum was clinically classified as asymptomatic, oligosymptomatic, and polysymptomatic and the parasite load range estimated.
The analysis of proteases expression by RT-PCR in the ear edge skin was also assessed, suggesting more transcripts of proteases in cDNA samples from polysymptomatic dogs than oligosymptomatic and asymptomatic ones.
Metalloprotease RT-PCR assays yielded products (202 bp) in all assessed cDNA dog samples.
In contrast, cysteine proteases transcripts (227 bp) had shown to be better detected in cDNA samples of polysymptomatic dogs, compared with cDNA samples from asymptomatic and oligosymptomatic dogs.
Predictive in silico assays suggested that secondary structures of metalloproteasee mRNAs can be more stable than cysteine proteases at the skin temperature of dogs.
Evidence is presented that during natural infection of dogs by L.
(L.) infantum, this parasite produces transcripts of metalloprotease and cysteine protease RNA in the skin from asymptomatic, oligosymptomatic, and polysymptomatic dogs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Veloso, Laura Barral& de Oliveira Cardoso, Flávia& dos Santos Charret, Karen& de Sá Silva, Matheus Pereira& de Castro Côrtes, Luzia Monteiro& Calabrese, Kátia da Silva…[et al.]. 2020. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1132630
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Veloso, Laura Barral…[et al.]. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1132630
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Veloso, Laura Barral& de Oliveira Cardoso, Flávia& dos Santos Charret, Karen& de Sá Silva, Matheus Pereira& de Castro Côrtes, Luzia Monteiro& Calabrese, Kátia da Silva…[et al.]. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1132630
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132630
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر