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Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein
المؤلفون المشاركون
Deng, Xichuan
Zhang, Chaoliang
Zhang, Kehan
Lu, Nan
He, Yonglin
Liu, Jia
Yang, Zhibang
Zhang, Guangyuan
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Human parainfluenza virus type 3 (HPIV3) is the main pathogen that causes respiratory infections in infants, young children, and the elderly.
Currently, there are no vaccines and effective anti-infective drugs.
Studying the replication and proliferation mechanism of HPIV3 is helpful for exploring the targets of anti-HPIV3 infection.
Matrix protein (M) and nucleocapsid protein (N) are two key structural proteins of HPIV3 that exert important functions in HPIV3 proliferation.
Herein, we aim to clarify the functional domains of M and N interaction.
HPIV3 M and N expression plasmids of pCAGGS-HA-M and pCAGGS-N-Myc/Flag, M C-terminal truncation mutant plasmids of pCAGGSHA-MΔC120, MΔC170, MΔC190, and MΔC210, and M C-terminal plasmid of pCAGGS-HA-MC190 and C-terminal deletion mutant plasmid of pCAGGS-MΔN143-182 were constructed.
By using immunoprecipitation, immunofluorescence, and virus-like particle (VLP) germination experiments, we found that N was encapsulated into M-mediated VLP through N and M interaction.
Moreover, the C-terminus of the M played a key role in the interaction between M and N.
The C-terminus of the M encapsulated the N into the VLP.
We finally determined that the 143-182 amino acids in the M were the functional regions that encapsulated the N into the M-mediated VLP.
Our findings confirmed the interaction between M and N and for the first time clarified that the 143-182 amino acid region in M was the functional region that interacted with N, which provides a molecular basis for exploring effective anti-HPIV3 targets.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deng, Xichuan& Zhang, Chaoliang& Zhang, Kehan& Lu, Nan& He, Yonglin& Liu, Jia…[et al.]. 2020. Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132633
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deng, Xichuan…[et al.]. Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1132633
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deng, Xichuan& Zhang, Chaoliang& Zhang, Kehan& Lu, Nan& He, Yonglin& Liu, Jia…[et al.]. Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132633
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132633
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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