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Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging
المؤلفون المشاركون
Dubois, Fatéméh
Bénard, Magalie
Jean-Jacques, Bastien
Schapman, Damien
Roberge, Hélène
Lebon, Alexis
Goux, Didier
Monterroso, Baptiste
Elie, Nicolas
Komuro, Hitoshi
Bazille, Céline
Levallet, Jérôme
Bergot, Emmanuel
Levallet, Guénaëlle
Galas, Ludovic
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-13
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
By allowing insured communication between cancer cells themselves and with the neighboring stromal cells, tunneling nanotubes (TNTs) are involved in the multistep process of cancer development from tumorigenesis to the treatment resistance.
However, despite their critical role in the biology of cancer, the study of the TNTs has been announced challenging due to not only the absence of a specific biomarker but also the fragile and transitory nature of their structure and the fact that they are hovering freely above the substratum.
Here, we proposed to review guidelines to follow for studying the structure and functionality of TNTs in tumoral neuroendocrine cells (PC12) and nontumorigenic human bronchial epithelial cells (HBEC-3, H28).
In particular, we reported how crucial is it (i) to consider the culture conditions (culture surface, cell density), (ii) to visualize the formation of TNTs in living cells (mechanisms of formation, 3D representation), and (iii) to identify the cytoskeleton components and the associated elements (categories, origin, tip, and formation/transport) in the TNTs.
We also focused on the input of high-resolution cell imaging approaches including Stimulated Emission Depletion (STED) nanoscopy, Transmitted and Scanning Electron Microscopies (TEM and SEM).
In addition, we underlined the important role of the organelles in the mechanisms of TNT formation and transfer between the cancer cells.
Finally, new biological models for the identification of the TNTs between cancer cells and stromal cells (liquid air interface, ex vivo, in vivo) and the clinical considerations will also be discussed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dubois, Fatéméh& Bénard, Magalie& Jean-Jacques, Bastien& Schapman, Damien& Roberge, Hélène& Lebon, Alexis…[et al.]. 2020. Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging. BioMed Research International،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1132670
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dubois, Fatéméh…[et al.]. Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging. BioMed Research International No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1132670
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dubois, Fatéméh& Bénard, Magalie& Jean-Jacques, Bastien& Schapman, Damien& Roberge, Hélène& Lebon, Alexis…[et al.]. Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1132670
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132670
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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