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lncRNA RP11-838N2.3 Promoted Cisplatin Resistance in Lung Adenocarcinoma
المؤلفون المشاركون
Chen, Jie
Jiang, Feng
Hu, Lijuan
Zhang, Fan
Wang, Junjun
Huang, Kate
Wang, Yumin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-15
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
The mechanism of RP11-838N2.3 promoting cisplatin resistance in lung adenocarcinoma (LAD) was unclear.
The RP11-838N2.3 expression level in cells and LAD tissues was detected by qPCR.
We constructed lentivirus-mediated GV303 overexpression and GV248 shRNA vector targeting RP11-838N2.3, then infected A549 and A549/DDP cell and furtherly analyzed cell biology.
High-throughput gene chip analysis showed that RP11-838N2.3 was significantly upregulated in A549/DDP (change fold=66.056595).
The qPCR results showed that the expression level of RP11-838N2.3 in A549/DDP cell was significantly higher than that in A549 cells (P<0.05), and the expression level of RP11-838N2.3 in LAD tissues was also significantly higher than that in adjacent tissues (P<0.05).
The expression level of RP11-838N2.3 in cisplatin-insensitive LAD tissues was also significantly higher than that in cisplatin-sensitive LAD tissues (P<0.05).
Survival analysis showed that OS (overall survival) and DFS (progression-free survival) of high RP11-838N2.3 expression in the cisplatin-sensitive or cisplatin-insensitive LAD group were lower (P<0.001 and P<0.001) than those of low RP11-838N2.3 expression in the cisplatin-sensitive or cisplatin-insensitive LAD group.
CCK8 showed that the OD450 value of RP11-838N2.3 overexpression increased significantly at 24 h, 48 h, and 72 h after transfection, while the knockdown of RP11-838N2.3 caused OD450 value at 24 h, 48 h, and 72 h after transfection significantly reduced, under the action of cisplatin that had the same trend (P<0.05).
The cell migration showed that the RP11-838N2.3 overexpression increased significantly migration activity and RP11-838N2.3 knockdown inhibited migration activity at 24 h, 48 h, and 72 h after transfection.
The same trend was also observed under the action of cisplatin (P<0.05).
The cell invasion showed that the invasion rate of RP11-838N2.3 overexpression increased significantly, while the invasion rate of RP11-838N2.3 knockdown decreased significantly, and the same trend was observed under the action of cisplatin (P<0.05).
Apoptosis results showed that the apoptosis rate of RP11-838N2.3 overexpressed cells decreased significantly and the apoptosis rate of RP11-838N2.3 knockdown cells increased significantly, and the same trend was also observed under the action of cisplatin (P<0.05).
However, the results of cell cycle showed that there was no significant difference in the proportion of cells in each phase of the cell cycle after RP11-838N2.3 overexpression or knockdown (P>0.05).RP11-838N2.3 was significantly upregulated in cisplatin-resistant cell and tissues of LAD.
RP11-838N2.3 could enhance the proliferation, migration, and invasion and inhibit apoptosis of LAD cisplatin-resistant cell.
So RP11-838N2.3 could enhance the cisplatin resistance of LAD cells and was a resistant lncRNA molecule.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Jie& Jiang, Feng& Hu, Lijuan& Zhang, Fan& Wang, Junjun& Huang, Kate…[et al.]. 2020. lncRNA RP11-838N2.3 Promoted Cisplatin Resistance in Lung Adenocarcinoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1132727
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Jie…[et al.]. lncRNA RP11-838N2.3 Promoted Cisplatin Resistance in Lung Adenocarcinoma. BioMed Research International No. 2020 (2020), pp.1-18.
https://search.emarefa.net/detail/BIM-1132727
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Jie& Jiang, Feng& Hu, Lijuan& Zhang, Fan& Wang, Junjun& Huang, Kate…[et al.]. lncRNA RP11-838N2.3 Promoted Cisplatin Resistance in Lung Adenocarcinoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1132727
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132727
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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