Simultaneous Determination of 13 Organic Acids in Liquid Culture Media of Edible Fungi Using High-Performance Liquid Chromatography
المؤلفون المشاركون
Zhao, Yan
Wang, Hong
Chen, Mingjie
Yu, Changxia
Wang, Yan
Cao, Hui
Li, Zhengpeng
Tang, Qingjiu
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The study aimed at detecting 13 organic acids (oxalic acid, maleic acid, citric acid, tartaric acid, malic acid, quinic acid, succinic acid, fumaric acid, formic acid, acetic acid, propionic acid, isobutyric acid, and butyric acid) by establishing a high-performance liquid chromatography (HPLC).
The analysis was performed using two sugar columns, i.e., SH1011 column and KC-811 column.
The optimal conditions were as follows: 4 mmol/L HClO4 solution as the eluent with UV-visible detector (210 nm), a flow rate of 1 mL/min at the temperature of 60°C, and the injection volume at 10 μL.
The results showed that all the calibration curves had excellent linearity (R2>0.9991) within the test ranges.
The RSD values of the thirteen analytes were lower than 2.94% at three levels, the recoveries were 91.9%-102.0%, the limit of detection (LOD) was between 0.05 and 10.63 μg/mL, and the quantification (LOQ) was between 0.10 and 19.53 μg/mL.
Finally, the proposed methodology was successfully applied for the analysis of organic acids in the culture medium of edible fungi.
In conclusion, the study findings proved that the method was sensitive, accurate, reproducible, and could be readily applied to analyze the organic acids in the samples.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Changxia& Wang, Yan& Cao, Hui& Zhao, Yan& Li, Zhengpeng& Wang, Hong…[et al.]. 2020. Simultaneous Determination of 13 Organic Acids in Liquid Culture Media of Edible Fungi Using High-Performance Liquid Chromatography. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132739
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Changxia…[et al.]. Simultaneous Determination of 13 Organic Acids in Liquid Culture Media of Edible Fungi Using High-Performance Liquid Chromatography. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132739
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Changxia& Wang, Yan& Cao, Hui& Zhao, Yan& Li, Zhengpeng& Wang, Hong…[et al.]. Simultaneous Determination of 13 Organic Acids in Liquid Culture Media of Edible Fungi Using High-Performance Liquid Chromatography. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132739
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132739
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر