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Downregulation of CRABP2 Inhibit the Tumorigenesis of Hepatocellular Carcinoma In Vivo and In Vitro
المؤلفون المشاركون
Chen, Qingmin
Tan, Ludong
Jin, Zhe
Liu, Yahui
Zhang, Ze
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-25
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Cellular retinoic acid-binding protein 2 (CRABP2) binds retinoic acid (RA) in the cytoplasm and transports it into the nucleus, allowing for the regulation of specific downstream signal pathway.
Abnormal expression of CRABP2 has been detected in the development of several tumors.
However, the role of CRABP2 in hepatocellular carcinoma (HCC) has never been revealed.
The current study aimed to investigate the role of CRABP2 in HCC and illuminate the potential molecular mechanisms.
The expression of CRABP2 in HCC tissues and cell lines was detected by western blotting and immunohistochemistry assays.
Our results demonstrated that the expression levels of CRABP2 in HCC tissues were elevated with the tumor stage development, and it was also elevated in HCC cell lines.
To evaluate the function of CRABP2, shRNA-knockdown strategy was used in HCC cells.
Cell proliferation, metastasis, and apoptosis were analyzed by CCK-8, EdU staining, transwell, and flow cytometry assays, respectively.
Based on our results, knockdown of CRABP2 by shRNA resulted in the inhibition of tumor proliferation, migration, and invasion in vitro, followed by increased tumor apoptosis-related protein expression and decreased ERK/VEGF pathway-related proteins expression.
CRABP2 silencing in HCC cells also resulted in the failure to develop tumors in vivo.
These results provide important insights into the role of CRABP2 in the development and development of HCC.
Based on our findings, CRABP2 may be used as a novel diagnostic biomarker, and regulation of CRABP2 in HCC may provide a potential molecular target for the therapy of HCC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Qingmin& Tan, Ludong& Jin, Zhe& Liu, Yahui& Zhang, Ze. 2020. Downregulation of CRABP2 Inhibit the Tumorigenesis of Hepatocellular Carcinoma In Vivo and In Vitro. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132992
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Qingmin…[et al.]. Downregulation of CRABP2 Inhibit the Tumorigenesis of Hepatocellular Carcinoma In Vivo and In Vitro. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1132992
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Qingmin& Tan, Ludong& Jin, Zhe& Liu, Yahui& Zhang, Ze. Downregulation of CRABP2 Inhibit the Tumorigenesis of Hepatocellular Carcinoma In Vivo and In Vitro. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132992
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132992
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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