Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4NF-κB Signaling Pathway
المؤلفون المشاركون
Zhang, Wei
Zhou, Ping
Jiang, Xiao
Fan, Zhe
Xu, Xingxin
Wang, Fei
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-20
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Tec kinase is an important mediator in inflammatory immune response that enhances the activity of neutrophils and macrophages.
However, information on its function in lipopolysaccharide- (LPS-) induced acute kidney injury (AKI) is limited.
This study is aimed at determining whether Tec kinase was a regulator in AKI.
An AKI model in mice was successfully established using intraperitoneal LPS.
Results showed that the serum levels of creatinine (Cr), blood urea nitrogen (BUN), and cystatin-C (Cys-C) increased after intraperitoneal LPS injection.
Renal tissue sustained significantly severe injury as measured by pathological scores.
Pretreatment with LFM-A13 improved the function of the kidney in mice and decreased the renal injury score.
Enzyme-linked immunosorbent assay showed that LFM-A13 significantly reduced the release of IL-1β and TNF-α in mice exposed to LPS.
LFM-A13 can evidently abrogate the expression of Tec protein, MyD88, TLR4, NF-κB p65, and Tec’s phosphorylated protein as determined by Western blot.
Immunohistochemistry analysis revealed that LFM-A13 markedly downregulated the expression of Tec kinase in renal tubular epithelial cells.
In vitro, Tec kinase protein was expressed highly in NRK-52E cells after LPS exposure.
Tec-siRNA also decreased IL-1β and TNF-α production and obviously abolished phospho-p65 and phospho-IκBα expression in NRK-52E cell stimulated by LPS; however, Tec-siRNA increased the IκBα level.
Altogether, these data suggested that Tec kinase can be a modulating protein in AKI through TLR4/NF-κB activation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Wei& Zhou, Ping& Jiang, Xiao& Fan, Zhe& Xu, Xingxin& Wang, Fei. 2020. Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4NF-κB Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1133024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Wei…[et al.]. Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4NF-κB Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1133024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Wei& Zhou, Ping& Jiang, Xiao& Fan, Zhe& Xu, Xingxin& Wang, Fei. Negative Regulation of Tec Kinase Alleviates LPS-Induced Acute Kidney Injury in Mice via theTLR4NF-κB Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1133024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1133024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر