Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots
المؤلفون المشاركون
Cai, JianPing
Liu, Xiangtao
Shang, Youjun
Yang, Shunli
Yuan, Li
Wu, Jinyan
Zhang, Jie
Podgórska, Katarzyna
Stepniewska, Katarzyna
Zafar Khan, Muhammad Umar
Yin, Shuanghui
Pejsak, Zygmunt
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Outbreak of classical swine fever (CSF) results in high mortality and thus causes severe economic losses in the swine industry.
Single-domain antibody (sdAb) is the smallest antigen-binding molecule derived from camelid heavy-chain antibodies and has the potential to be used as a molecular probe for detection of CSF virus (CSFV).
In this study, two sdAb fragments against the E2 antigen of CSFV were obtained, expressed in vitro.
The functional characteristics analysis indicated that the recombinant sdAbE2-1 and sdAbE2-2 have excellent binding activity, specificity, and high affinity with equilibrium constant value of 3.34 × 10−7 and 1.35 × 10−8 M to E2 protein.
Then, sdAbE2s were conjugated with quantum dots (QD)/AF488 to synthesize two molecular probes for imaging CSFV distribution in cells.
The sdAbE2-1 was also labeled with carboxyl-magnetic beads to construct immunomagnetic nanobeads (IMNBs) able to capture CSFV virions and recombinant E2 protein.
QD/AF455-sdAbE2s probes colocalised with CSFV virions in swine testis cells, and IMNBs were used as a detection template and proved to bind specifically with CSFV virions and E2 protein.
The selected sdAb fragments and sdAb-based molecular probes may be used for the rapid identification of CSFV during field outbreaks and for research on CSFV and host interactions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Shunli& Yuan, Li& Shang, Youjun& Wu, Jinyan& Liu, Xiangtao& Zhang, Jie…[et al.]. 2020. Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133068
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Shunli…[et al.]. Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1133068
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Shunli& Yuan, Li& Shang, Youjun& Wu, Jinyan& Liu, Xiangtao& Zhang, Jie…[et al.]. Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133068
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1133068
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر