Astragalus membranaceus–Derived Anti-Programmed Death-1 Monoclonal Antibodies with Immunomodulatory Therapeutic Effects against Tumors
المؤلفون المشاركون
Lee, Yu-Ching
Tsai, Keng-Chang
Pan, Shiow-Lin
Chang, Fu-Ling
Lin, Tsai-Yu
Yang, Tz-Wen
Lo, Yan-Ni
Chen, Wang-Chuan
Chang, Jui-Hsien
Lu, Mei-Kuang
Chiou, Chun-Tang
Chen, Po-Hung
Yen, Yun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-04
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Astragalus membranaceus polysaccharide (APS) components are main ingredients of TCM and have proven efficacy to activate T cells and B cells, enhancing immunity in humans.
In this study, elevated cytokine and anti-PD-1 antibody titers were found in mice after immunization with APS.
Therefore, phage-display technology was utilized to isolate specific anti-programmed death-1 (PD-1) antibodies from mice stimulated by APS and to confirm whether the isolated anti-PD-1 antibody could inhibit the interaction of PD-1 with the programmed death-ligand 1 (PD-L1), resulting in tumor growth inhibition.
The isolated single-chain fragment variable (scFv) S12 exhibited the highest binding affinity of 20 nM to PD-1, completed the interaction between PD-1 and PD-L1, and blocked the effect of PD-L1-induced T cell exhaustion in peripheral blood mononuclear cells in vitro.
In the animal model, the tumor growth inhibition effect after scFv S12 treatment was approximately 48%.
However, meaningful synergistic effects were not observed when scFv S12 was used as a cotreatment with ixabepilone.
Moreover, this treatment caused a reduction in the number of tumor-associated macrophages in the tumor tissue.
These experimental results indirectly indicate the ability of APS to induce specific antibodies associated with the immune checkpoint system and the potential benefits for improving immunity in humans.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chang, Fu-Ling& Tsai, Keng-Chang& Lin, Tsai-Yu& Yang, Tz-Wen& Lo, Yan-Ni& Chen, Wang-Chuan…[et al.]. 2020. Astragalus membranaceus–Derived Anti-Programmed Death-1 Monoclonal Antibodies with Immunomodulatory Therapeutic Effects against Tumors. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133151
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chang, Fu-Ling…[et al.]. Astragalus membranaceus–Derived Anti-Programmed Death-1 Monoclonal Antibodies with Immunomodulatory Therapeutic Effects against Tumors. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1133151
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chang, Fu-Ling& Tsai, Keng-Chang& Lin, Tsai-Yu& Yang, Tz-Wen& Lo, Yan-Ni& Chen, Wang-Chuan…[et al.]. Astragalus membranaceus–Derived Anti-Programmed Death-1 Monoclonal Antibodies with Immunomodulatory Therapeutic Effects against Tumors. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133151
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1133151
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر