Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis
المؤلفون المشاركون
Qian, Zhixiang
Zhao, Dan
Yin, Yu
Zhu, Hui
Chen, Daijie
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Worldwide interest in the use of functional foods containing probiotic bacteria such as Lactobacillus and Bifidobacterium for health promotion and disease prevention has increased significantly.
Probiotics have demonstrated beneficial properties including strengthening the body’s natural defense system, inhibiting the growth of pathogenic bacteria, and regulating mental activity, but their effects on the human vagina have not been fully elucidated.
The primary purpose of our study was to isolate Lactobacillus strains from old yogurt, a traditional dairy product, and investigate their probiotic potential with respect to the human vaginal system.
Four Lactobacillus plantarum (L.
plantarum) strains, named ZX1, ZX2, ZX27, and ZX69, were isolated from the yogurt samples.
Simultaneously, we used a commercial Lactobacillus strain (Lactobacillus delbrueckii DM8909) as a control strain.
We tested the antimicrobial activity of Lactobacillus isolates against Escherichia coli and Gardnerella vaginalis by agar spot and well diffusion tests.
Then, we tested the antibiotic susceptibility of the 5 strains by using the minimal inhibitory concentration method.
We attempted to detect possible bacteriocin genes by PCR sequencing technique.
Using a chemically defined medium simulating genital tract secretions, we found that the selected Lactobacillus strains could alter the expression of known virulence genes in Gardnerella vaginalis.
Bacteriocins derived from these isolated strains had potent antibacterial activity against G.
vaginalis and E.
coli, with the most effective activity observed in the case of ZX27.
In addition, all strains including the L.
delbrueckii DM8909 were positive for the presence of the plantaricin cluster of genes described in L.
plantarum C11.
The tested stains possessed the pln gene indicating that one of the antibacterial agents was plantaricin.
We assume that the production of antimicrobial substances such as bacteriocins induce G.
vaginalis to upregulate antimicrobial resistance genes.
The new isolated strains have bacteriocin-related genes and can change the antimicrobial resistance gene transcription of G.
vaginalis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qian, Zhixiang& Zhao, Dan& Yin, Yu& Zhu, Hui& Chen, Daijie. 2020. Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133288
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qian, Zhixiang…[et al.]. Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1133288
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qian, Zhixiang& Zhao, Dan& Yin, Yu& Zhu, Hui& Chen, Daijie. Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133288
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1133288
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر