Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis

المؤلفون المشاركون

Liang, Min
Chen, Zhao
Xiao, Xijuan
Xin, Caihong
Zhang, Yuqin
Xu, Huiyong
Wu, Buling
Fang, Fuchun
Qiu, Wei
Yan, Jie

المصدر

BioMed Research International

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-12-10

دولة النشر

مصر

عدد الصفحات

14

التخصصات الرئيسية

الطب البشري

الملخص EN

Background.

The repair of dental pulp injury relies on the odontogenic differentiation of dental pulp stem cells (DPSCs).

To better understand the odontogenic differentiation of DPSCs and identify proteins involved in this process, tandem mass tags (TMTs) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied to compare the proteomic profiles of induced and control DPSCs.

Methods.

The proteins expressed during osteogenic differentiation of human DPSCs were profiled using the TMT method combined with LC-MS/MS analysis.

The identified proteins were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.

Then, a protein-protein interaction (PPI) network was constructed.

Two selected proteins were confirmed by western blotting (WB) analysis.

Results.

A total of 223 proteins that were differentially expressed were identified.

Among them, 152 proteins were significantly upregulated and 71 were downregulated in the odontogenic differentiation group compared with the control group.

On the basis of biological processes in GO, the identified proteins were mainly involved in cellular processes, metabolic processes, and biological regulation, which are connected with the signaling pathways highlighted by KEGG pathway analysis.

PPI networks showed that most of the differentially expressed proteins were implicated in physical or functional interaction.

The protein expression levels of FBN1 and TGF-β2 validated by WB were consistent with the proteomic analysis.

Conclusions.

This is the first proteomic analysis of human DPSC odontogenesis using a TMT method.

We identified many new differentially expressed proteins that are potential targets for pulp-dentin complex regeneration and repair.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Xiao, Xijuan& Xin, Caihong& Zhang, Yuqin& Yan, Jie& Chen, Zhao& Xu, Huiyong…[et al.]. 2020. Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis. BioMed Research International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1133512

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Xiao, Xijuan…[et al.]. Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis. BioMed Research International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1133512

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Xiao, Xijuan& Xin, Caihong& Zhang, Yuqin& Yan, Jie& Chen, Zhao& Xu, Huiyong…[et al.]. Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1133512

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1133512