Tumor-Suppressive Function of lncRNA-MEG3 in Glioma Cells by Regulating miR-6088SMARCB1 Axis

الملخص EN

Objective.

Mounting evidence has elaborated the implication of long noncoding RNAs (lncRNAs) in tumorigenesis of several cancers, including glioma.

However, little was known about the mechanism of lncRNA maternally expressed gene 3 (MEG3) in the development and progression of glioma.

This work is designed to explore the effect of MEG3 on glioma progression and its possible mechanism.

Methods.

Expressions of lncRNA-MEG3 and SMARCB1 were detected in human glioblastoma U87 and U251 cell lines.

Gain and loss of function of MEG3 or/and miR-6088 was performed in U87 and U251 cells to observe its effect on cell proliferation and migration as well as on epithelial-mesenchymal transition (EMT) related markers.

Luciferase reporter gene assay was employed to inspect the interactions among MEG3, miR-6088, and SMARCB1.

Results.

MEG3 and SMARCB1 expressions were downregulated in glioma cells.

Transfection of pcDNA3.1-MEG3 or pcDNA3.1-SMARCB1 plasmids could clearly block cell proliferation, migration, and EMT progression.

MEG3 functions as a sponge for miR-6088, while SMARCB1 is a downstream protein of miR-6088.

Transfection of miR-6088 mimic or si-SMARCB1 could obviously reverse the favorable effect of pcDNA3.1-MEG3 on glioma progression.

Conclusion.

Collectively, the evidence in this study indicated that MEG3 was downregulated in glioma cells and inhibited proliferation and migration of glioma cells via regulating miR-6088/SMARCB1 axis.